Sequential nitrification/mixotrophic denitrification process in single bioreactor: Performance and microbiology of two types of sequencing batch bioreactors

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dc.coverageDOI: 10.1016/j.biortech.2025.133200
dc.creatorPalominos, Nicolás
dc.creatorPagés-Díaz, Jhosané
dc.creatorTello, Mario
dc.creatorGuerrero, Lorna
dc.creatorHuiliñir, Cesar
dc.date2025
dc.date.accessioned05-01-2026 18:17
dc.date.available05-01-2026 18:17
dc.description<p>The integration of sequential nitrification/mixotrophic denitrification (SNMD) in a single bioreactor is rarely reported. This study adapted an industrial inoculum to SNMD in a single bioreactor configuration, comparing a conventional sequencing batch reactor (SBR) and a zeolite-carrier SBR (SBBR) over 276 days across three phases: sequential nitrification heterotrophic denitrification (SNHD) adaptation, increased TAN loading, and SNMD with sulfide (HS<sup>-</sup>-S) addition. Under SNHD conditions, both systems achieved &gt; 99 % TAN removal with complete NO<sub>3</sub><sup>–</sup>-N reduction. After HS<sup>-</sup>-S addition, NO<sub>2</sub><sup>–</sup>-N accumulated, yet denitrification recovered after 90 days. Under SNMD, TAN removal averaged 93.4 ± 5.6 % in the SBR and 90.0 ± 10.6 % in the SBBR (p &gt; 0.05). While performance remained similar, zeolite influenced microbial community composition, particularly under sulfur-driven conditions. Therefore, the SNMD process was successfully developed in a single reactor, with or without zeolite, which only affected microbial abundance.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/2df31fba-c6fc-41b9-800a-36c7b3e41611
dc.languageeng
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourcevol.438 (2025) p.133200
dc.subjectLong-term performance
dc.subjectNitrogen removal
dc.subjectSulfide
dc.subjectZeolite
dc.subjectSDG 7 - Affordable and Clean Energy
dc.titleSequential nitrification/mixotrophic denitrification process in single bioreactor: Performance and microbiology of two types of sequencing batch bioreactorseng
dc.typeArticleeng
dc.typeArtículospa
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Doctorado en Ciencias de la Ingeniería (DOCI)