Sequential nitrification/mixotrophic denitrification process in single bioreactor: Performance and microbiology of two types of sequencing batch bioreactors
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<p>The integration of sequential nitrification/mixotrophic denitrification (SNMD) in a single bioreactor is rarely reported. This study adapted an industrial inoculum to SNMD in a single bioreactor configuration, comparing a conventional sequencing batch reactor (SBR) and a zeolite-carrier SBR (SBBR) over 276 days across three phases: sequential nitrification heterotrophic denitrification (SNHD) adaptation, increased TAN loading, and SNMD with sulfide (HS<sup>-</sup>-S) addition. Under SNHD conditions, both systems achieved > 99 % TAN removal with complete NO<sub>3</sub><sup>–</sup>-N reduction. After HS<sup>-</sup>-S addition, NO<sub>2</sub><sup>–</sup>-N accumulated, yet denitrification recovered after 90 days. Under SNMD, TAN removal averaged 93.4 ± 5.6 % in the SBR and 90.0 ± 10.6 % in the SBBR (p > 0.05). While performance remained similar, zeolite influenced microbial community composition, particularly under sulfur-driven conditions. Therefore, the SNMD process was successfully developed in a single reactor, with or without zeolite, which only affected microbial abundance.</p>
Keywords
Long-term performance, Nitrogen removal, Sulfide, Zeolite, SDG 7 - Affordable and Clean Energy