Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma

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dc.coverageDOI: 10.1111/jop.13455
dc.creatorda Costa, Bruno Cesar
dc.creatorDourado, Mauricio Rocha
dc.creatorde Moraes, Everton Freitas
dc.creatorPanini, Luana Marí
dc.creatorElseragy, Amr
dc.creatorTéo, Fábio Haach
dc.creatorGuimarães, Gustavo Narvaes
dc.creatorMachado, Renato Assis
dc.creatorRisteli, Maija
dc.creatorGurgel Rocha, Clarissa Araujo
dc.creatorParanaíba, Lívia Máris Ribeiro
dc.creatorGonzález-Arriagada, Wilfredo Alejandro
dc.creatorda Silva, Sabrina Daniela
dc.creatorRangel, Ana Lucia Carrinho Ayroza
dc.creatorMarques, Marcelo Rocha
dc.creatorSalo, Tuula
dc.creatorColetta, Ricardo D.
dc.date2023
dc.date.accessioned2025-11-18T19:42:37Z
dc.date.available2025-11-18T19:42:37Z
dc.description<p>BACKGROUND: The expression of heat-shock protein 47 (HSP47) has been linked to collagen synthesis control and implicated in fibrotic disorders, but more recent studies have demonstrated its role in solid tumors. In this study, we explored the prognostic impact of HSP47 in oral squamous cell carcinomas (OSCC) and determined the in vitro effects of its loss-of-function on viability, proliferation, migration, invasion, and resistance to cisplatin of OSCC cells.</p><p>METHODS: The HSP47 expression in tumor samples was assessed by immunohistochemistry in two independent cohorts totaling 339 patients with OSCC, and protein levels were associated with clinicopathological features and survival outcomes. The OSCC cell lines HSC3 and SCC9 were transduced with lentivirus expressing short hairpin RNA to stably silence HSP47 and used in assays to measure cellular viability, proliferation, migration, and invasion.</p><p>RESULTS: HSP47 was overexpressed in OSCC samples, and its overexpression was significantly and independently associated with poor disease-specific survival and shortened disease-free survival in both OSCC cohorts. The knockdown of HSP47 showed no effects on cell viability or cisplatin sensitivity, but impaired significantly proliferation, migration, and invasion of OSCC cells, with stronger effects on SCC9 cells.</p><p>CONCLUSION: Our results show a significant prognostic impact of HSP47 overexpression in OSCC and reveal that HSP47 inhibition impairs the proliferation, migration, and invasion of OSCC cells. HSP47 may represent a potential therapeutic target for OSCC.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/efaf956a-5574-44fc-9674-7089ca99361f
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/52431
dc.languageeng
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.sourcevol.52 (2023) nr.7 p.601-609
dc.subjectSERPINH1
dc.subjectinvasion
dc.subjectoral cancer
dc.subjectprognosis
dc.subjectproliferation
dc.subjectSDG 3 - Good Health and Well-being
dc.titleOverexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinomaeng
dc.typeArticleeng
dc.typeArtículospa
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