Impact of local and systemic antimicrobials on leukocyte- and platelet rich fibrin: an in vitro study

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dc.coverageDOI: 10.1038/s41598-022-06473-4
dc.creatorSiawasch, S. A.M.
dc.creatorAndrade, C.
dc.creatorCastro, A. B.
dc.creatorTeughels, W.
dc.creatorTemmerman, A.
dc.creatorQuirynen, M.
dc.date2022
dc.date.accessioned2025-11-18T19:48:28Z
dc.date.available2025-11-18T19:48:28Z
dc.description<p>The aim of this study was to evaluate the effect of local and systemic administration of antimicrobials to leukocyte- and platelet-rich fibrin (L-PRF). For part A, 16 tubes of venous blood were collected from each of eight systemically healthy subjects. Prior to blood centrifugation, 12 of the 16 tubes were injected with 0.125 ml, 0.25 ml or 0.50 ml metronidazole solution. One set of L-PRF membranes was used to assess the release of vascular endothelial growth factor AB, platelet-derived growth factor, transforming growth factor beta 1, and bone morphogenetic protein 2 at indicated time points. The metronidazole release over time by L-PRF membranes was also evaluated. The remaining L-PRF membranes were placed on the surface of agar plates inoculated with three different periodontal pathogens to determine their antibacterial activity. For part B, another six subjects were enrolled with three subjects taking 2 g amoxicillin and three subjects 500 mg metronidazole as prophylaxis prior to a periodontal treatment. Before and 2 h after consuming one of the prescribed antimicrobials, three tubes of blood were collected for preparing L-PRF membranes. These membranes were used to measure the antibacterial activity against periodontal pathogens. No statistically significant difference could be found in the release of growth factors between L-PRF membranes with and without incorporation of metronidazole solution. The release of metronidazole could be detected up to day 3, however with the highest concentration during the first 4 h. This concentration was dose dependent. The antibacterial capacity of L-PRF membranes increased significantly for both the systemic intake, and after the addition of metronidazole solution to the blood tubes before centrifugation, the latter again dose dependent. The antibacterial capacity of L-PRF against the periodontal pathogens tested can significantly be enhanced by the addition of antimicrobials, without disadvantage for the release of growth factors.</p>eng
dc.descriptionThe aim of this study was to evaluate the effect of local and systemic administration of antimicrobials to leukocyte- and platelet-rich fibrin (L-PRF). For part A, 16 tubes of venous blood were collected from each of eight systemically healthy subjects. Prior to blood centrifugation, 12 of the 16 tubes were injected with 0.125 ml, 0.25 ml or 0.50 ml metronidazole solution. One set of L-PRF membranes was used to assess the release of vascular endothelial growth factor AB, platelet-derived growth factor, transforming growth factor beta 1, and bone morphogenetic protein 2 at indicated time points. The metronidazole release over time by L-PRF membranes was also evaluated. The remaining L-PRF membranes were placed on the surface of agar plates inoculated with three different periodontal pathogens to determine their antibacterial activity. For part B, another six subjects were enrolled with three subjects taking 2 g amoxicillin and three subjects 500 mg metronidazole as prophylaxis prior to a periodontal treatment. Before and 2 h after consuming one of the prescribed antimicrobials, three tubes of blood were collected for preparing L-PRF membranes. These membranes were used to measure the antibacterial activity against periodontal pathogens. No statistically significant difference could be found in the release of growth factors between L-PRF membranes with and without incorporation of metronidazole solution. The release of metronidazole could be detected up to day 3, however with the highest concentration during the first 4 h. This concentration was dose dependent. The antibacterial capacity of L-PRF membranes increased significantly for both the systemic intake, and after the addition of metronidazole solution to the blood tubes before centrifugation, the latter again dose dependent. The antibacterial capacity of L-PRF against the periodontal pathogens tested can significantly be enhanced by the addition of antimicrobials, without disadvantage for the release of growth factors.spa
dc.identifierhttps://investigadores.uandes.cl/en/publications/fc6d6f1e-42c3-42af-923a-03dcbd4d9ab5
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/55577
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.12 (2022) nr.1 p.1-10
dc.subjectAdult
dc.subjectAged
dc.subjectAmoxicillin/pharmacology
dc.subjectAnti-Infective Agents/pharmacokinetics
dc.subjectAntibiotic Prophylaxis/methods
dc.subjectBacteria/drug effects
dc.subjectHealthy Volunteers
dc.subjectHumans
dc.subjectIntercellular Signaling Peptides and Proteins/metabolism
dc.subjectMembranes/metabolism
dc.subjectMetronidazole/pharmacokinetics
dc.subjectMiddle Aged
dc.subjectPlatelet-Rich Fibrin/drug effects
dc.subjectYoung Adult
dc.subjectAmoxicillin/pharmacology
dc.subjectAnti-Infective Agents/pharmacokinetics
dc.subjectMetronidazole/pharmacokinetics
dc.subjectPlatelet-rich fibrin
dc.titleImpact of local and systemic antimicrobials on leukocyte- and platelet rich fibrin: an in vitro studyeng
dc.typeArticleeng
dc.typeArtículospa
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