Urinary Extracellular Vesicles as a Source of NGAL for Diabetic Kidney Disease Evaluation in Children and Adolescents With Type 1 Diabetes Mellitus

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dc.coverageDOI: 10.3389/fendo.2021.654269
dc.creatorUgarte, Francisca
dc.creatorSantapau, Daniela
dc.creatorGallardo, Vivian
dc.creatorGarfias, Carolina
dc.creatorYizmeyián, Anahí
dc.creatorVillanueva, Soledad
dc.creatorSepúlveda, Carolina
dc.creatorRocco, Jocelyn
dc.creatorPasten, Consuelo
dc.creatorUrquidi, Cinthya
dc.creatorCavada, Gabriel
dc.creatorSan Martin, Pamela
dc.creatorCano, Francisco
dc.creatorIrarrázabal, Carlos E.
dc.date2022
dc.date.accessioned2025-11-18T19:41:47Z
dc.date.available2025-11-18T19:41:47Z
dc.description<p>Background: Tubular damage has a role in Diabetic Kidney Disease (DKD). We evaluated the early tubulointerstitial damage biomarkers in type-1 Diabetes Mellitus (T1DM) pediatric participants and studied the correlation with classical DKD parameters. Methods: Thirty-four T1DM and fifteen healthy participants were enrolled. Clinical and biochemical parameters [Glomerular filtration Rate (GFR), microalbuminuria (MAU), albumin/creatinine ratio (ACR), and glycated hemoglobin A1c (HbA1c)] were evaluated. Neutrophil gelatinase-associated lipocalin (NGAL), Hypoxia-inducible Factor-1α (HIF-1α), and Nuclear Factor of Activated T-cells-5 (NFAT5) levels were studied in the supernatant (S) and the exosome-like extracellular vesicles (E) fraction from urine samples. Results: In the T1DM, 12% had MAU &gt;20 mg/L, 6% ACR &gt;30 mg/g, and 88% had eGFR &gt;140 ml/min/1.72 m<sup>2</sup>. NGAL in the S (NGAL-S) or E (NGAL-E) fraction was not detectable in the control. The NGAL-E was more frequent (p = 0.040) and higher (p = 0.002) than NGAL-S in T1DM. The T1DM participants with positive NGAL had higher age (p = 0.03), T1DM evolution (p = 0.03), and serum creatinine (p = 0.003) than negative NGAL. The NGAL-E correlated positively with tanner stage (p = 0.0036), the median levels of HbA1c before enrollment (p = 0.045) and was independent of ACR, MAU, and HbA1c at the enrollment. NFAT5 and HIF-1α levels were not detectable in T1DM or control. Conclusion: Urinary exosome-like extracellular vesicles could be a new source of early detection of tubular injury biomarkers of DKD in T1DM patients.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/639eff32-a800-4d73-bb35-ff92bb0c5a6f
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/52003
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.12 (2022) date: 2022-01-03
dc.subjectNGAL
dc.subjectchildren
dc.subjectdiabetic kidney disease
dc.subjecttype 1 diabetes mellitus
dc.subjecturinary extracellular vesicles
dc.subjectSDG 3 - Good Health and Well-being
dc.titleUrinary Extracellular Vesicles as a Source of NGAL for Diabetic Kidney Disease Evaluation in Children and Adolescents With Type 1 Diabetes Mellituseng
dc.typeArticleeng
dc.typeArtículospa
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