1400W Prevents Renal Injury in the Renal Cortex But Not in the Medulla in a Murine Model of Ischemia and Reperfusion Injury

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dc.coverageDOI: 10.33594/000000577
dc.creatorPasten, Consuelo
dc.creatorLozano, Mauricio
dc.creatorMéndez, Gonzalo P.
dc.creatorIrarrázabal, Carlos E.
dc.date2022
dc.date.accessioned2025-11-18T19:42:09Z
dc.date.available2025-11-18T19:42:09Z
dc.description<p>Background/Aims: Acute kidney injury (AKI) carries high morbidity and mortality, and the inducible nitric oxide synthase (iNOS) is a potential molecular target to prevent kidney dysfunction. In previous work, we reported that the pharmacological inhibitions of iNOS before ischemia/reperfusion (I/R) attenuate the I/R-induced AKI in mice. Here, we study the iNOS inhibitor 1400W [N-(3-(Aminomethyl)benzyl] acetamide, which has been described to be much more specific to iNOS inhibition than other compounds. Methods: We used 30 minutes of bilateral renal ischemia, followed by 24 hours of reperfusion in Balb/c mice. 1400w (10 mg/ kg i.p) was applied before I/R injury. We measured the expression of elements associated with kidney injury, inflammation, macrophage polarization, mesenchymal transition, and nephrogenic genes by qRT-PCR in the renal cortex and medulla. The Periodic Acid-Schiff (PAS) was used to study the kidney morphology. Results: Remarkably, we found that 1400W affects the renal cortex and medulla in different ways. Thus, in the renal cortex, 1400W prevented the I/R-upregulation of 1. NGAL, Clusterin, and signs of morphological damage; 2. IL-6 and TNF-α; 3. TGF-β; 4. M2(Arg1, Erg2, cMyc) and M1(CD38, Fpr2) macrophage polarization makers; and 5. Vimentin and FGF2 levels but not in the renal medulla. Conclusion: 1400W conferred protection in the kidney cortex compared to the kidney medulla. The present investigation provides relevant information to understand the opportunity to use 1400W as a therapeutic approach in AKI treatment.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/336878e7-40c7-4caa-9d61-e0ff049bfa96
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/52200
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.56 (2022) date: 2022-10-19 nr.5 p.573-586
dc.subject1400W
dc.subjectInflammation
dc.subjectIschemia/reperfusion injury
dc.subjectMacrophages polarization
dc.title1400W Prevents Renal Injury in the Renal Cortex But Not in the Medulla in a Murine Model of Ischemia and Reperfusion Injuryeng
dc.typeArticleeng
dc.typeArtículospa
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