Active mmp-8 quantitative test as an adjunctive tool for early diagnosis of periodontitis

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dc.coverageDOI: 10.3390/diagnostics11081503
dc.creatorHernández, Marcela
dc.creatorBaeza, Mauricio
dc.creatorRäisänen, Ismo T.
dc.creatorContreras, Johanna
dc.creatorTervahartiala, Taina
dc.creatorChaparro, Alejandra
dc.creatorSorsa, Timo
dc.creatorHernández-Ríos, Patricia
dc.date2021
dc.date.accessioned2025-11-18T19:48:05Z
dc.date.available2025-11-18T19:48:05Z
dc.description<p>Periodontitis is a host-mediated bacterial disease that affects the tooth attachment apparatus. Metalloproteinase-8 (MMP-8), a validated biomarker, could aid in clinical diagnosis. This study aimed to evaluate the diagnostic performance of active (a) MMP-8 immunotest versus total (t) MMP-8 ELISA for quantitative real-time diagnosis and assessment of periodontitis severity at the site level. Gingival crevicular fluid (GCF) was sampled from 30 healthy, 42 mild, and 59 severe periodontitis sites from thirty-one volunteers. MMP-8 concentrations were determined by time-resolved immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using the STATA package. Both active and total MMP-8-based methods discriminated among sites according to periodontal diagnosis and severity, with a positive correlation between the two tests (p &lt; 0.001). (a) MMP-8 models showed the best performance in receiver operating characteristic (ROC) curves to discriminate between healthy and periodontitis sites (area under the curve [AUC] = 0.89), while (t) MMP-8 demonstrated a high diagnostic precision in the detection of mild from severe periodontitis sites (AUC ≥ 0.80). The use of (a) MMP-8 and (t) MMP-8 could represent a useful adjunctive tool for periodontitis diagnosis and severity. These results support the applicability of new point-of-care methods in the monitoring of high-risk periodontal patients.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/19ae59aa-9ebf-4a64-bc9c-fe77a8ce603d
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/55378
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.11 (2021) date: 2021-08-20 nr.8 p.1-11
dc.subjectBiomarkers
dc.subjectDiagnosis
dc.subjectGingival crevicular fluid
dc.subjectMatrix metalloproteinase-8
dc.subjectPeriodontal disease
dc.subjectPeriodontitis
dc.titleActive mmp-8 quantitative test as an adjunctive tool for early diagnosis of periodontitiseng
dc.typeArticleeng
dc.typeArtículospa
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