Influence of Topography and Composition of Commercial Titanium Dental Implants on Cell Adhesion of Human Gingiva-Derived Mesenchymal Stem Cells: An In Vitro Study

Simple item page
dc.coverageDOI: 10.3390/ijms242316686
dc.creatorCampos-Bijit, Vanessa
dc.creatorInostroza, Nicolás Cohn
dc.creatorOrellana, Rocío
dc.creatorRivera, Alejandro
dc.creatorMarttens, Alfredo Von
dc.creatorCortez, Cristian
dc.creatorCovarrubias, Cristian
dc.date2023
dc.date.accessioned2025-11-18T19:43:40Z
dc.date.available2025-11-18T19:43:40Z
dc.descriptionThe topography and composition of dental implant surfaces directly impact mesenchymal cell adhesion, proliferation, and differentiation, crucial aspects of achieving osseointegration. However, cell adhesion to biomaterials is considered a key step that drives cell proliferation and differentiation. The aim of this study was to characterize characterize the topography and composition of commercial titanium dental implants manufactured with different surface treatments (two sandblasted/acid-etched (SLA) (INNO Implants, Busan, Republic of Korea; BioHorizonsTM, Oceanside, CA, USA) and two calcium phosphate (CaP) treated (Biounite®, Berazategui, Argentina; Zimmer Biomet, Inc., Warsaw, IN, USA)) and to investigate their influence on the process of cell adhesion in vitro. A smooth surface implant (Zimmer Biomet, Inc.) was used as a control. For that, high-resolution methodologies such as scanning electron microscopy (SEM), X-ray dispersive spectroscopy (EDX), laser scanning confocal microscopy (LSCM), and atomic force microscopy (AFM) were employed. Protein adsorption and retromolar gingival mesenchymal stem cells (GMSCs) adhesion to the implant surfaces were evaluated after 48 h. The adherent cells were examined by SEM and LSCM for morphologic and quantitative analyses. ANOVA and Tukey tests (α = 0.05) were employed to determine statistical significance. SEM revealed that INNO, BioHorizonsTM, and Zimmer implants have an irregular surface, whereas Biounite® has a regular topography consisting of an ordered pattern. EDX confirmed a calcium and phosphate layer on the Biounite® and Zimmer surfaces, and AFM exhibited different roughness parameters. Protein adsorption and cell adhesion were detected on all the implant surfaces studied. However, the Biounite® implant with CaP and regular topography showed the highest protein adsorption capacity and density of adherent GMSCs. Although the Zimmer implant also had a CaP treatment, protein and cell adhesion levels were lower than those observed with Biounite®. Our findings indicated that the surface regularity of the implants is a more determinant factor in the cell adhesion process than the CaP treatment. A regular, nanostructured, hydrophilic, and moderately rough topography generates a higher protein adsorption capacity and thus promotes more efficient cell adhesion.eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/a1b8687d-47d2-406c-a4f0-7b66ad14ea58
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/53002
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.24 (2023) date: 2023-11-24 nr.23 p.1-22
dc.subjectcell adhesion
dc.subjectdental implant surface
dc.subjectprotein adsorption
dc.subjectretromolar gingiva-derived
dc.subjectmesenchymal stem cells
dc.subjecttopography
dc.titleInfluence of Topography and Composition of Commercial Titanium Dental Implants on Cell Adhesion of Human Gingiva-Derived Mesenchymal Stem Cells: An In Vitro Studyeng
dc.typeArticleeng
dc.typeArtículospa
Files
Collections
PURE