NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration

Simple item page
dc.coverageDOI: 10.1038/s41467-021-26422-5
dc.creatorLaplace-Builhé, Béryl
dc.creatorBarthelaix, Audrey
dc.creatorAssou, Said
dc.creatorBohaud, Candice
dc.creatorPratlong, Marine
dc.creatorSeverac, Dany
dc.creatorTejedor, Gautier
dc.creatorLuz-Crawford, Patricia
dc.creatorNguyen-Chi, Mai
dc.creatorMathieu, Marc
dc.creatorJorgensen, Christian
dc.creatorDjouad, Farida
dc.date2021
dc.date.accessioned2025-11-18T19:41:38Z
dc.date.available2025-11-18T19:41:38Z
dc.description<p>Fish species, such as zebrafish (Danio rerio), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3-positive cell population within the regenerating fin. Genetic depletion of these foxd3-positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3-positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3-positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.</p>eng
dc.descriptionFish species, such as zebrafish (Danio rerio), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3-positive cell population within the regenerating fin. Genetic depletion of these foxd3-positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3-positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3-positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.spa
dc.identifierhttps://investigadores.uandes.cl/en/publications/29b2917f-28e7-4aa4-ada2-891a62039da3
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/51910
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.12 (2021) date: 2021-11-03 nr.1 p.6336
dc.subjectAnimal Fins
dc.subjectAnimals
dc.subjectCell Proliferation
dc.subjectForkhead Transcription Factors
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectDevelopmental; Genes
dc.subjecterbB
dc.subjectLarva
dc.subjectMacrophages
dc.subjectNeural Crest
dc.subjectNeuregulin-1
dc.subjectRegeneration
dc.subjectSignal Transduction
dc.subjectStem Cells
dc.subjectZebrafish
dc.subjectZebrafish Proteins
dc.titleNRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regenerationeng
dc.typeArticleeng
dc.typeArtículospa
Files
Collections
PURE