Impact of platelet-derived mitochondria transfer in the metabolic profiling and progression of metastatic MDA-MB-231 human triple-negative breast cancer cells

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dc.coverageDOI: 10.3389/fcell.2023.1324158
dc.creatorCereceda, Lucas
dc.creatorCardenas, J. Cesar
dc.creatorKhoury, Maroun
dc.creatorSilva-Pavez, Eduardo
dc.creatorHidalgo, Yessia
dc.date2024
dc.date.accessioned2025-11-18T19:42:55Z
dc.date.available2025-11-18T19:42:55Z
dc.description<p>Introduction: An active role of platelets in the progression of triple-negative breast cancer (TNBC) cells has been described. Even the role of platelet-derived extracellular vesicles on the migration of MDA-MB-231 cells has been reported. Interestingly, upon activation, platelets release functional mitochondria into the extracellular environment. However, the impact of these platelet-derived mitochondria on the metabolic properties of MDA-MB-231 cells remains unclear. Methods: MDA-MB-231 and MDA-MB-231-Rho-0 cells were co-cultured with platelets, which were isolated from donor blood. Mitochondrial transfer was assessed through confocal microscopy and flow cytometry, while metabolic analyses were conducted using a Seahorse XF HS Mini Analyzer. The mito-chondrial DNA (mtDNA) copy number was determined via quantitative PCR (qPCR) following platelet co-culture. Finally, cell proliferation and colony formation assay were performed using crystal violet staining. Results and Discussion: We have shown that platelet-derived mitochondria are internalized by MDA-MB-231 cells in co-culture with platelets, increasing ATP production, oxygen (O<sub>2</sub>) consumption rate (OCR), cell proliferation, and metabolic adaptability. Additionally, we observed that MDA-MB-231 cells depleted from mtDNA restore cell proliferation in uridine/pyruvate-free cell culture medium and mitochondrial O<sub>2</sub> consumption after co-culture with platelets, indicating a reconstitution of mtDNA facilitated by platelet-derived mitochondria. In conclusion, our study provides new insights into the role of platelet-derived mitochondria in the metabolic adaptability and progression of metastatic MDA-MB-231 TNBC cells.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/03669829-0654-4978-bfdd-0e76c298da9e
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/52606
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.11 (2024) date: 2024-01-14 p.1324158
dc.subjectcancer
dc.subjectmetabolic adaptability
dc.subjectmitochondria transfer
dc.subjectplatelet-derived mitochondria
dc.subjectplatelets
dc.subjectSDG 3 - Good Health and Well-being
dc.titleImpact of platelet-derived mitochondria transfer in the metabolic profiling and progression of metastatic MDA-MB-231 human triple-negative breast cancer cellseng
dc.typeArticleeng
dc.typeArtículospa
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