Conformational and Structural Changes in Chickpea Proteins Caused by Simulated Salivary Alterations in the Elderly

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dc.coverageDOI: 10.3390/foods12193668
dc.creatorContardo, Ingrid
dc.creatorGuzmán, Fanny
dc.creatorEnrione, Javier
dc.date2023
dc.date.accessioned2025-11-18T19:42:49Z
dc.date.available2025-11-18T19:42:49Z
dc.description<p>The impact of salivary alterations on chickpea protein structure in the elderly has not been well documented. This study aimed to understand the role of simulated salivary alterations in the conformational properties and secondary structure of the chickpea protein isolate (CPI). Whey protein isolate (WPI) was used as the reference. Protein dispersions (10%) were subjected to in vitro oral processing under simulated salivary conditions in both the elderly and adult subjects. Proteins and their oral counterparts were characterized in terms of their composition, charge, size, solubility, water absorption, molecular weight (MW), and secondary structure (Circular Dichroism and Raman spectroscopy). Under condition of simulated oral digestion in the elderly population, the ordered secondary protein structure was significantly affected, decreasing α-helix by ~36% and ~29% in CPI and WPI compared to the control (adult) population, respectively. An increase in the unordered random coil state was observed. These results could be attributed to an increase in electrolytes in the salivary composition. The structure of CPI is more stable than that of WPI because of its higher MW, more rigid structure, less charged surface, and different amino acid compositions. This study is meaningful in understanding how alterations in the elderly oral system affect protein conformation and is expected to improve the understanding of plant-based protein digestibility.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/927451e7-2ede-4344-9837-58b711a09d96
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/52542
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.12 (2023) date: 2023-10-05 nr.19 p.1-14
dc.subjectchickpea proteins
dc.subjectcircular dichroism
dc.subjectelderly salivary alterations
dc.subjectoral electrolytes
dc.subjectsecondary structure
dc.subjectwhey proteins
dc.titleConformational and Structural Changes in Chickpea Proteins Caused by Simulated Salivary Alterations in the Elderlyeng
dc.typeArticleeng
dc.typeArtículospa
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