Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers

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dc.coverageDOI: 10.1007/s10295-019-02186-2
dc.creatorPacheco, Nicolas
dc.creatorOrellana-Saez, Matias
dc.creatorPepczynska, Marzena
dc.creatorEnrione, Javier
dc.creatorBassas-Galia, Monica
dc.creatorBorrero-de Acuña, Jose M.
dc.creatorZacconi, Flavia C.
dc.creatorMarcoleta, Andrés E.
dc.creatorPoblete-Castro, Ignacio
dc.date2019
dc.date.accessioned2025-11-18T19:53:50Z
dc.date.available2025-11-18T19:53:50Z
dc.description<p>Extreme environments are a unique source of microorganisms encoding metabolic capacities that remain largely unexplored. In this work, we isolated two Antarctic bacterial strains able to produce poly(3-hydroxyalkanoates) (PHAs), which were classified after 16S rRNA analysis as Pseudomonas sp. MPC5 and MPC6. The MPC6 strain presented nearly the same specific growth rate whether subjected to a temperature of 4 °C 0.18 (1/h) or 30 °C 0.2 (1/h) on glycerol. Both Pseudomonas strains produced high levels of PHAs and exopolysaccharides from glycerol at 4 °C and 30 °C in batch cultures, an attribute that has not been previously described for bacteria of this genus. The MPC5 strain produced the distinctive medium-chain-length-PHA whereas Pseudomonas sp. MPC6 synthesized a novel polyoxoester composed of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate). Batch bioreactor production of PHAs in MPC6 resulted in a titer of 2.6 (g/L) and 1.3 (g/L), accumulating 47.3% and 34.5% of the cell dry mass as PHA, at 30 and 4 °C, respectively. This study paves the way for using Antarctic Pseudomonas strains for biosynthesizing novel PHAs from low-cost substrates such as glycerol and the possibility to carry out the bioconversion process for biopolymer synthesis without the need for temperature control.</p>eng
dc.descriptionExtreme environments are a unique source of microorganisms encoding metabolic capacities that remain largely unexplored. In this work, we isolated two Antarctic bacterial strains able to produce poly(3-hydroxyalkanoates) (PHAs), which were classified after 16S rRNA analysis as Pseudomonas sp. MPC5 and MPC6. The MPC6 strain presented nearly the same specific growth rate whether subjected to a temperature of 4 °C 0.18 (1/h) or 30 °C 0.2 (1/h) on glycerol. Both Pseudomonas strains produced high levels of PHAs and exopolysaccharides from glycerol at 4 °C and 30 °C in batch cultures, an attribute that has not been previously described for bacteria of this genus. The MPC5 strain produced the distinctive medium-chain-length-PHA whereas Pseudomonas sp. MPC6 synthesized a novel polyoxoester composed of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate). Batch bioreactor production of PHAs in MPC6 resulted in a titer of 2.6 (g/L) and 1.3 (g/L), accumulating 47.3% and 34.5% of the cell dry mass as PHA, at 30 and 4 °C, respectively. This study paves the way for using Antarctic Pseudomonas strains for biosynthesizing novel PHAs from low-cost substrates such as glycerol and the possibility to carry out the bioconversion process for biopolymer synthesis without the need for temperature control.spa
dc.identifierhttps://investigadores.uandes.cl/en/publications/c61949f1-9a90-4d24-8207-680aa53ca9f2
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/58454
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.46 (2019) date: 2019-08-01 nr.8 p.1139-1153
dc.subjectAntarctic Pseudomonas
dc.subjectExopolysaccharide
dc.subjectGlycerol
dc.subjectLow temperature
dc.subjectPoly(3-hydroxyalkanoates)
dc.subjectPsychrophiles
dc.subjectAntarctic Pseudomonas
dc.subjectExopolysaccharide
dc.subjectGlycerol
dc.subjectLow temperature
dc.subjectPoly(3-hydroxyalkanoates)
dc.subjectPsychrophiles
dc.titleExploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymerseng
dc.typeArticleeng
dc.typeArtículospa
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