Designing a gelatin/chitosan/hyaluronic acid biopolymer using a thermophysical approach for use in tissue engineering

Simple item page
dc.coverageDOI: 10.1007/s00449-013-0971-x
dc.creatorEnrione, Javier
dc.creatorDíaz-Calderón, Paulo
dc.creatorWeinstein-Oppenheimer, Caroline R.
dc.creatorSánchez, Elizabeth
dc.creatorFuentes, Miguel A.
dc.creatorBrown, Donald I.
dc.creatorHerrera, Hugo
dc.creatorAcevedo, Cristian A.
dc.date2013
dc.date.accessioned2025-11-18T19:47:31Z
dc.date.available2025-11-18T19:47:31Z
dc.description<p>Cell culture on biopolymeric scaffolds has provided treatments for tissue engineering. Biopolymeric mixtures based on gelatin (Ge), chitosan (Ch) and hyaluronic acid (Ha) have been used to make scaffolds for wound healing. Thermal and physical properties of scaffolds prepared with Ge, Ch and Ha were characterized. Thermal characterization was made by using differential scanning calorimetry (DSC), and physical characterization by gas pycnometry and scanning electron microscopy. The effects of Ge content and cross-linking on thermophysical properties were evaluated by means of a factorial experiment design (central composite face centered). Gelatin content was the main factor that affects the thermophysical properties (microstructure and thermal transitions) of the scaffold. The effect of Ge content of the scaffolds for tissue engineering was studied by seeding skin cells on the biopolymers. The cell attachment was not significantly modified at different Ge contents; however, the cell growth rate increased linearly with the decrease of the Ge content. This relationship together with the thermophysical characterization may be used to design scaffolds for tissue engineering.</p>eng
dc.descriptionCell culture on biopolymeric scaffolds has provided treatments for tissue engineering. Biopolymeric mixtures based on gelatin (Ge), chitosan (Ch) and hyaluronic acid (Ha) have been used to make scaffolds for wound healing. Thermal and physical properties of scaffolds prepared with Ge, Ch and Ha were characterized. Thermal characterization was made by using differential scanning calorimetry (DSC), and physical characterization by gas pycnometry and scanning electron microscopy. The effects of Ge content and cross-linking on thermophysical properties were evaluated by means of a factorial experiment design (central composite face centered). Gelatin content was the main factor that affects the thermophysical properties (microstructure and thermal transitions) of the scaffold. The effect of Ge content of the scaffolds for tissue engineering was studied by seeding skin cells on the biopolymers. The cell attachment was not significantly modified at different Ge contents; however, the cell growth rate increased linearly with the decrease of the Ge content. This relationship together with the thermophysical characterization may be used to design scaffolds for tissue engineering.spa
dc.identifierhttps://investigadores.uandes.cl/en/publications/64562dc1-fe6b-4164-96b7-d7215e8fa86d
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/55085
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.36 (2013) nr.12 p.1947-1956
dc.subjectBiopolymeric scaffold
dc.subjectChitosan
dc.subjectGelatin
dc.subjectHyaluronic acid
dc.subjectThermophysical properties
dc.subjectTissue engineering
dc.titleDesigning a gelatin/chitosan/hyaluronic acid biopolymer using a thermophysical approach for use in tissue engineeringeng
dc.typeArticleeng
dc.typeArtículospa
Files
Collections
PURE