Novel core–shell nanocapsules for the tunable delivery of bioactive rhEGF: Formulation, characterization and cytocompatibility studies

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dc.coverageDOI: 10.1166/jbt.2015.1385
dc.creatorRamasamy, Thiruganesh
dc.creatorKim, Jong Oh
dc.creatorYong, Chul Soon
dc.creatorUmadevi, Kandasamy
dc.creatorRana, Deepti
dc.creatorJiménez, Constanza
dc.creatorCampos, Javier
dc.creatorRamalingam, Murugan
dc.creatorHaidar, Ziyad S.
dc.date2015
dc.date.accessioned2025-11-18T19:46:47Z
dc.date.available2025-11-18T19:46:47Z
dc.descriptionAll rights reserved. Epidermal growth factor (EGF) has been demonstrated to play a crucial role in the regeneration of skin. However, the topical application of the cytokine is limited by several key drawbacks, such as hair loss, chemical instability, need for supra-physiological (un-safe) dosages, photo-degradation as well as high cost; leading to patient non-compliance. The aim of the present study was to formulate a novel hybrid tunable delivery system in the form of a core–shell nanocapsules-based suspension. Nanocapsules were composed of cationic solid lipid nanoparticles (SLN)-based core and a customizable bi-layered shell structure produced by the layer-by-layer self-assembly of alternative coatings of anionic hyaluronan (HA) and cationic chitosan (CH). The resulted core–shell based nanocapsules were found to be physically stable, spherical, monodisperse, hydrophilic and cytocompatible in nature (<280 nm, cationic). The modulated release of low, safe and effective bioactive dosages of EGF is demonstrated. The system offers compartments for protein entrapment including the aqueous core and within the polyelectrolyte layers in the shell. To the best of knowledge, this is the first report describing the build-up of a tunable biopolymeric shell on a SLN core for the controlled/predictable delivery of bioactive recombinant human EGF with potential utility in skin regeneration and wound healing.eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/d45a657e-7112-4586-b05b-58ff633016fd
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/54705
dc.languageeng
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourcevol.5 (2015) date: 2015-09-01 nr.9 p.730-743
dc.subjectChitosan
dc.subjectCore-Shell nanocapsules
dc.subjectProtein delivery
dc.subjectRecombinant human epidermal growth factor
dc.subjectSkin regeneration
dc.subjectSolid lipid nanoparticles
dc.subjectChitosan
dc.subjectCore-Shell nanocapsules
dc.subjectProtein delivery
dc.subjectRecombinant human epidermal growth factor
dc.subjectSkin regeneration
dc.subjectSolid lipid nanoparticles
dc.subjectSDG 3 - Good Health and Well-being
dc.titleNovel core–shell nanocapsules for the tunable delivery of bioactive rhEGF: Formulation, characterization and cytocompatibility studieseng
dc.typeArticleeng
dc.typeArtículospa
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