A Role for mir-26a in Stress: A Potential sEV Biomarker and Modulator of Excitatory Neurotransmission

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dc.coverageDOI: 10.3390/cells9061364
dc.creatorLafourcade, Carlos Andrés
dc.creatorFernández, Anllely
dc.creatorRamírez, Juan Pablo
dc.creatorCorvalán, Katherine
dc.creatorCarrasco, Miguel Ángel
dc.creatorIturriaga, Andrés
dc.creatorBátiz, Luis Federico
dc.creatorLuarte, Alejandro
dc.creatorWyneken, Ursula
dc.date2020
dc.date.accessioned2025-11-18T19:53:37Z
dc.date.available2025-11-18T19:53:37Z
dc.description<p>Stress is a widespread problem in today's societies, having important consequences on brain function. Among the plethora of mechanisms involved in the stress response at the molecular level, the role of microRNAs (miRNAs) is beginning to be recognized. The control of gene expression by these noncoding RNAs makes them essential regulators of neuronal and synaptic physiology, and alterations in their levels have been associated with pathological conditions and mental disorders. In particular, the excitatory (i.e., glutamate-mediated) neurotransmission is importantly affected by stress. Here, we found that loss of miR-26a-5p (miR-26a henceforth) function in primary hippocampal neurons increased the frequency and amplitude of miniature excitatory currents, as well as the expression levels of the excitatory postsynaptic scaffolding protein PSD95. Incubation of primary hippocampal neurons with corticosterone downregulated miR-26a, an effect that mirrored our in vivo results, as miR-26a was downregulated in the hippocampus as well as in blood serum-derived small extracellular vesicles (sEVs) of rats exposed to two different stress paradigms by movement restriction (i.e., stress by restraint in cages or by complete immobilization in bags). Overall, these results suggest that miR-26a may be involved in the generalized stress response and that a stress-induced downregulation of miR-26a could have long-term effects on glutamate neurotransmission.</p>eng
dc.descriptionStress is a widespread problem in today's societies, having important consequences on brain function. Among the plethora of mechanisms involved in the stress response at the molecular level, the role of microRNAs (miRNAs) is beginning to be recognized. The control of gene expression by these noncoding RNAs makes them essential regulators of neuronal and synaptic physiology, and alterations in their levels have been associated with pathological conditions and mental disorders. In particular, the excitatory (i.e., glutamate-mediated) neurotransmission is importantly affected by stress. Here, we found that loss of miR-26a-5p (miR-26a henceforth) function in primary hippocampal neurons increased the frequency and amplitude of miniature excitatory currents, as well as the expression levels of the excitatory postsynaptic scaffolding protein PSD95. Incubation of primary hippocampal neurons with corticosterone downregulated miR-26a, an effect that mirrored our in vivo results, as miR-26a was downregulated in the hippocampus as well as in blood serum-derived small extracellular vesicles (sEVs) of rats exposed to two different stress paradigms by movement restriction (i.e., stress by restraint in cages or by complete immobilization in bags). Overall, these results suggest that miR-26a may be involved in the generalized stress response and that a stress-induced downregulation of miR-26a could have long-term effects on glutamate neurotransmission.spa
dc.identifierhttps://investigadores.uandes.cl/en/publications/b10f4319-a1e5-4d22-be4d-d0bacb6be50e
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/58338
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.9 (2020) date: 2020-06-01 nr.6
dc.subjectextracellular vesicles
dc.subjecthippocampal neurons
dc.subjectmiRNAs
dc.subjectstress
dc.subjectsynapses
dc.subjectExtracellular vesicles
dc.subjecthippocampal neurons
dc.subjectmiRNAs
dc.subjectStress
dc.subjectsynapses
dc.titleA Role for mir-26a in Stress: A Potential sEV Biomarker and Modulator of Excitatory Neurotransmissioneng
dc.typeArticleeng
dc.typeArtículospa
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