Isolation of Intestinal Macrophage Subpopulations for High-Quality Total RNA Purification in Zebrafish

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dc.coverageDOI: 10.3390/mps7030043
dc.creatorDel Río-Jay, Yalén
dc.creatorBarthelaix, Audrey
dc.creatorReyes-Martínez, Cristian
dc.creatorDuperray, Christophe
dc.creatorSolis-Cascante, Camila J.
dc.creatorHidalgo, Yessia
dc.creatorLuz-Crawford, Patricia
dc.creatorDjouad, Farida
dc.creatorFeijoo, Carmen G.
dc.date2024
dc.date.accessioned2025-11-18T19:55:23Z
dc.date.available2025-11-18T19:55:23Z
dc.description<p>Intestinal macrophages have been poorly studied in fish, mainly due to the lack of specific molecular markers for their identification and isolation. To address this gap, using the zebrafish Tg(mpeg1:EGFP) transgenic line, we developed a fluorescence-activated cell sorting strategy (FACS) that allows us to isolate different intestinal macrophage subpopulations, based on GFP expression and morphological differences. Also, we achieved the purification of high-quality total RNA from each population to perform transcriptomic analysis. The complete strategy comprises three steps, including intestine dissection and tissue dissociation, the isolation of each intestinal macrophage population via FACS, and the extraction of total RNA. To be able to characterize molecularly different macrophage subpopulations and link them to their functional properties will allow us to unravel intestinal macrophage biology.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/3d87584e-a8a7-457f-81e3-532fb28d3753
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/59233
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.7 (2024) nr.3
dc.subjectintestinal macrophage
dc.subjectmRNA
dc.subjectzebrafish
dc.titleIsolation of Intestinal Macrophage Subpopulations for High-Quality Total RNA Purification in Zebrafisheng
dc.typeArticleeng
dc.typeArtículospa
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