Pulpal Responses to Leukocyte- and Platelet-Rich Plasma Treatment in Mouse Models for Immediate and Intentionally Delayed Tooth Replantation

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dc.coverageDOI: 10.3390/app142311358
dc.creatorQuispe-Salcedo, Angela
dc.creatorSuzuki-Barrera, Kiyoko
dc.creatorZapata-Sifuentes, Mauricio
dc.creatorWatanabe, Taisuke
dc.creatorKawase, Tomoyuki
dc.creatorOhshima, Hayato
dc.date2024
dc.date.accessioned2025-11-18T19:50:42Z
dc.date.available2025-11-18T19:50:42Z
dc.description<p>Featured Application: The goal of leukocyte and platelet-rich plasma (L-PRP) research is to apply it in clinical practice to treat traumatic dental events, such as tooth avulsions. Topical treatment with L-PRP before tooth replantation may provide a better environment for pulpal healing for severely injured teeth. This study aimed to evaluate the effect of leukocyte and platelet-rich plasma (L-PRP) on the pulpal healing process following immediate and intentionally delayed tooth replantation in mice. After the maxillary first molars of 3-week-old mice were extracted, the teeth were immersed for 1 min [immediate reimplantation (IR)] or 30 min [intentionally delayed reimplantation (IDR)] in phosphate-buffered saline (PBS) solution. The alveolar socket was filled with or without 1.5 μL of L-PRP [experimental or control groups (EG or CG)] followed by tooth replantation. Samples were collected from day 1 to week 4 after the operation, processed for histology, and evaluated by immunohistochemistry for Nestin and Ki-67 expression. Quantitative analysis revealed positive Nestin staining during pulpal healing in the EG at week 1 following IR and week 2 following IDR. Hard tissue deposition was significantly increased in the EG after IR at week 2. Cell proliferation was higher in the EG compared with that in the CG at week 1 and significantly decreased in the coronal pulp of the EG after the IDR at week 2. Our data suggest that treatment with L-PRP may have a positive effect on pulpal healing, even in teeth replanted after an extended extra-oral period.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/fd685163-683a-4c99-9c5e-7a95067f89cf
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/56758
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.14 (2024) nr.23
dc.subjectcell proliferation
dc.subjectdental pulp
dc.subjectimmunohistochemistry
dc.subjectmice
dc.subjectplatelet-rich plasma
dc.subjecttooth replantation
dc.titlePulpal Responses to Leukocyte- and Platelet-Rich Plasma Treatment in Mouse Models for Immediate and Intentionally Delayed Tooth Replantationeng
dc.typeArticleeng
dc.typeArtículospa
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