eNOS-dependent S-nitrosylation of the NF-κB subunit p65 has neuroprotective effects

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dc.coverageDOI: 10.1038/s41419-020-03338-4
dc.creatorCaviedes, Ariel
dc.creatorMaturana, Barbara
dc.creatorCorvalán, Katherina
dc.creatorEngler, Alexander
dc.creatorGordillo, Felipe
dc.creatorVaras-Godoy, Manuel
dc.creatorSmalla, Karl Heinz
dc.creatorBatiz, Luis Federico
dc.creatorLafourcade, Carlos
dc.creatorKaehne, Thilo
dc.creatorWyneken, Úrsula
dc.date2021
dc.date.accessioned2025-11-18T19:40:47Z
dc.date.available2025-11-18T19:40:47Z
dc.description<p>Cell death by glutamate excitotoxicity, mediated by N-methyl-d-aspartate (NMDA) receptors, negatively impacts brain function, including but not limited to hippocampal neurons. The NF-κB transcription factor (composed mainly of p65/p50 subunits) contributes to neuronal death in excitotoxicity, while its inhibition should improve cell survival. Using the biotin switch method, subcellular fractionation, immunofluorescence, and luciferase reporter assays, we found that NMDA-stimulated NF-κB activity selectively in hippocampal neurons, while endothelial nitric oxide synthase (eNOS), an enzyme expressed in neurons, is involved in the S-nitrosylation of p65 and consequent NF-κB inhibition in cerebrocortical, i.e., resistant neurons. The S-nitro proteomes of cortical and hippocampal neurons revealed that different biological processes are regulated by S-nitrosylation in susceptible and resistant neurons, bringing to light that protein S-nitrosylation is a ubiquitous post-translational modification, able to influence a variety of biological processes including the homeostatic inhibition of the NF-κB transcriptional activity in cortical neurons exposed to NMDA receptor overstimulation.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/a049173f-ef5f-4913-ba91-300617796034
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/51471
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.12 (2021) date: 2021-01-04 nr.1 p.4
dc.subjectNitric-oxide
dc.subjectActivation
dc.subjectExpression
dc.subjectNeurons
dc.subjectKinase
dc.subjectSDG 3 - Good Health and Well-being
dc.titleeNOS-dependent S-nitrosylation of the NF-κB subunit p65 has neuroprotective effectseng
dc.typeArticleeng
dc.typeArtículospa
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