Long Noncoding RNA TALAM1 Is a Transcriptional Target of the RUNX2 Transcription Factor in Lung Adenocarcinoma

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dc.coverageDOI: 10.3390/cimb45090447
dc.creatorBermúdez, Gisella
dc.creatorBernal, Camila
dc.creatorOtalora, Andrea
dc.creatorSanchez, Paula
dc.creatorNardocci, Gino
dc.creatorCañas, Alejandra
dc.creatorLopez-Kleine, Liliana
dc.creatorMontecino, Martín
dc.creatorRojas, Adriana
dc.date2023
dc.date.accessioned2025-11-18T19:42:48Z
dc.date.available2025-11-18T19:42:48Z
dc.description<p>Background: Lung cancer is the leading cause of cancer death worldwide. It has been reported that genetic and epigenetic factors play a crucial role in the onset and evolution of lung cancer. Previous reports have shown that essential transcription factors in embryonic development contribute to this pathology. Runt-related transcription factor (RUNX) proteins belong to a family of master regulators of embryonic developmental programs. Specifically, RUNX2 is the master transcription factor (TF) of osteoblastic differentiation, and it can be involved in pathological conditions such as prostate, thyroid, and lung cancer by regulating apoptosis and mesenchymal–epithelial transition processes. In this paper, we identified TALAM1 (Metastasis Associated Lung Adenocarcinoma Transcript 1) as a genetic target of the RUNX2 TF in lung cancer and then performed functional validation of the main findings. Methods: We performed ChIP-seq analysis of tumor samples from a patient diagnosed with lung adenocarcinoma to evaluate the target genes of the RUNX2 TF. In addition, we performed shRNA-mediated knockdown of RUNX2 in this lung adenocarcinoma cell line to confirm the regulatory role of RUNX2 in TALAM1 expression. Results: We observed RUNX2 overexpression in cell lines and primary cultured lung cancer cells. Interestingly, we found that lncRNA TALAM1 was a target of RUNX2 and that RUNX2 exerted a negative regulatory effect on TALAM1 transcription.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/9aff94a5-3b17-4839-ab6b-dfa258ed9ce7
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/52535
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.45 (2023) nr.9 p.7075-7086
dc.subjectChIP-seq
dc.subjectRUNX2
dc.subjectTALAM1
dc.subjectlung cancer
dc.subjectSDG 3 - Good Health and Well-being
dc.titleLong Noncoding RNA TALAM1 Is a Transcriptional Target of the RUNX2 Transcription Factor in Lung Adenocarcinomaeng
dc.typeArticleeng
dc.typeArtículospa
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