Regulatory T cells administration reduces anxiety-like behavior in mice submitted to chronic restraint stress

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dc.coverageDOI: 10.3389/fncel.2024.1406832
dc.creatorCepeda, Yamila
dc.creatorElizondo-Vega, Roberto
dc.creatorGarrido, Camila
dc.creatorTobar, Catalina
dc.creatorAraneda, Matías
dc.creatorOliveros, Patricia
dc.creatorOrdenes, Patricio
dc.creatorCarril, Claudio
dc.creatorVidal, Pía M.
dc.creatorLuz-Crawford, Patricia
dc.creatorGarcía-Robles, María A.
dc.creatorOyarce, Karina
dc.date2024
dc.date.accessioned2025-11-18T19:50:11Z
dc.date.available2025-11-18T19:50:11Z
dc.description<p>Background: Major depression disorder (MDD) and anxiety are common mental disorders that significantly affect the quality of life of those who suffer from them, altering the person’s normal functioning. From the biological perspective, the most classical hypothesis explaining their occurrence relies on neurotransmission and hippocampal excitability alterations. However, around 30% of MDD patients do not respond to medication targeting these processes. Over the last decade, the involvement of inflammatory responses in depression and anxiety pathogenesis has been strongly acknowledged, opening the possibility of tackling these disorders from an immunological point of view. In this context, regulatory T cells (Treg cells), which naturally maintain immune homeostasis by suppressing inflammation could be promising candidates for their therapeutic use in mental disorders. Methods: To test this hypothesis, C57BL/6 adult male mice were submitted to classical stress protocols to induce depressive and anxiety-like behavior; chronic restriction stress (CRS), and chronic unpredictable stress (CUS). Some of the stressed mice received a single adoptive transfer of Treg cells during stress protocols. Mouse behavior was analyzed through the open field (OFT) and forced swim test (FST). Blood and spleen samples were collected for T cell analysis using cell cytometry, while brains were collected to study changes in microglia by immunohistochemistry. Results: Mice submitted to CRS and CUS develop anxiety and depressive-like behavior, and only CRS mice exhibit lower frequencies of circulating Treg cells. Adoptive transfer of Treg cells decreased anxiety-like behavior in the OFT only in CRS model, but not depressive behavior in FST in neither of the two models. In CRS mice, Treg cells administration lowered the number of microglia in the hippocampus, which increased due this stress paradigm, and restored its arborization. However, in CUS mice, Treg cells administration increased microglia number with no significant effect on their arborization. Conclusion: Our results for effector CD4<sup>+</sup> T cells in the spleen and microglia number and morphology in the hippocampus add new evidence in favor of the participation of inflammatory responses in the development of depressive and anxiety-like behavior and suggest that the modulation of key immune cells such as Treg cells, could have beneficial effects on these disorders.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/6d6aaddf-4caa-46e2-a57c-96dc7794e938
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/56488
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.18 (2024)
dc.subjectanxiety
dc.subjectchronic restraint stress (CRS)
dc.subjectchronic unpredictable mild stress (CUS)
dc.subjectdepression
dc.subjectinflammation
dc.subjectneuroinflammation
dc.subjectregulatory T cells (Treg cells)
dc.titleRegulatory T cells administration reduces anxiety-like behavior in mice submitted to chronic restraint stresseng
dc.typeArticleeng
dc.typeArtículospa
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