Delivery of affordable and scalable encapsulated allogenic/autologous mesenchymal stem cells in coagulated platelet poor plasma for dental pulp regeneration

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dc.coverageDOI: 10.1038/s41598-021-02118-0
dc.creatorAngelopoulos, Ioannis
dc.creatorTrigo, Cesar
dc.creatorOrtuzar, Maria Ignacia
dc.creatorCuenca, Jimena
dc.creatorBrizuela, Claudia
dc.creatorKhoury, Maroun
dc.date2022
dc.date.accessioned2025-11-18T19:41:45Z
dc.date.available2025-11-18T19:41:45Z
dc.description<p>The main goal of regenerative endodontics procedures (REPs) is to revitalize teeth by the regeneration of healthy dental pulp. In this study, we evaluated the potential of combining a natural and accessible biomaterial based on Platelet Poor Plasma (PPP) as a support for dental pulp stem cells (DPSC) and umbilical cord mesenchymal stem cells (UC-MSC). A comparison study between the two cell sources revealed compatibility with the PPP based scaffold with differences noted in the proliferation and angiogenic properties in vitro. Additionally, the release of growth factors including VEGF, HGF and DMP-1, was detected in the media of cultured PPP and was enhanced by the presence of the encapsulated MSCs. Dentin-Discs from human molars were filled with PPP alone or with MSCs and implanted subcutaneously for 4 weeks in mice. Histological analysis of the MSC-PPP implants revealed a newly formed dentin-like structure evidenced by the expression of Dentin sialophosphoprotein (DSPP). Finally, DPSC induced more vessel formation around the dental discs. This study provides evidence of a cost-effective, xenofree scaffold that is compatible with either autologous or allogenic strategy for dental pulp regeneration. This attempt if successfully implemented, could make REPs treatment widely accessible, contributing in improving global health conditions.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/490aa103-78e4-43c3-9a13-b00c13189b2e
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/51990
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.12 (2022) nr.1 p.1-15
dc.subjectAnimals
dc.subjectDental Pulp/cytology
dc.subjectFemale
dc.subjectHumans
dc.subjectInfant, Newborn
dc.subjectMale
dc.subjectMesenchymal Stem Cells/physiology
dc.subjectMice
dc.subjectMicroscopy, Electron, Scanning
dc.subjectNeovascularization, Physiologic
dc.subjectPlasma
dc.subjectRegeneration
dc.subjectTissue Scaffolds
dc.subjectUmbilical Cord/cytology
dc.subjectYoung Adult
dc.subjectSDG 3 - Good Health and Well-being
dc.titleDelivery of affordable and scalable encapsulated allogenic/autologous mesenchymal stem cells in coagulated platelet poor plasma for dental pulp regenerationeng
dc.typeArticleeng
dc.typeArtículospa
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