Efectos de la irradiación con láser de diodo de 970 m sobre la morfología, la proliferación y la diferenciación de las células madre mesenquimales gingivales

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dc.coverageDOI: 10.17126/joralres.2023.008
dc.creatorOrtiz, Javiera
dc.creatorMatamala, Fernando
dc.creatorAndrade, Catherine
dc.creatorGutierrez, David
dc.creatorInostroza Silva, Carolina
dc.date2023
dc.date.accessioned2025-11-18T19:49:26Z
dc.date.available2025-11-18T19:49:26Z
dc.description<p>Objective: The objective of this study was to investigate the morphology, proliferation, and differentiation of gingival mesenchymal stem cells (GMSCs) irradiated with a 970 nm Diode Laser (LLLT). It is essential to validate the efficacy of treatment, optimize irradiation conditions and guarantee the safety and quality of stem cells for future use in dental applications. Materials and Methods: GMSCs were cultured in standard conditions and irradiated with a Diode laser (970 nm, 0.5W) with an energy density of 9J/cm2. Cell proliferation was assessed with the WST-1 proliferation kit. GMSCs were differentiated into chondrogenic and osteogenic lineages. Cell morphology was performed with Hematoxylin/eosin staining, and quantitative nuclear analysis was done. Cell viability was monitored with trypan blue testing. Results: GMSCs subjected to irradiation demonstrated a significant increase in proliferation at 72 hours compared to the non-irradiated controls (p=0.027). This indicates that the 970 nm diode laser has a stimulatory effect on the proliferation of GMSCs. LLLT-stimulated GMSCs exhibited the ability to differentiate into chondrogenic and osteogenic lineages. A substantial decrease in cell viability was observed 24 hours after irradiation (p=0.024). However, after 48 hours, the cell viability recovered without any significant differences. This indicates that there might be a temporary negative impact on cell viability immediately following irradiation, but the cells were able to recover and regain their viability over time. Conclusions: This study support that irradiation with a 970 nm diode laser could stimulate the proliferation of GMSCs, maintain their ability to differentiate into chondrogenic and osteogenic lineages, and has minimal impact on the mor-phological characteristics of the cells. These results support the potential use of NIR Lasers in combination with GMSCs as a promising strategy for dental treatments.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/8a16b657-3953-46f5-8153-17f51537bd3d
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/56091
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.12 (2023) date: 2023-04-04 nr.1 p.86-99
dc.subjectCell Proliferation
dc.subjectGingiva
dc.subjectMesenchymal stem cells
dc.subjectPhotobiomodulation
dc.subjectSafety
dc.subjectsemiconductor lasers
dc.titleEfectos de la irradiación con láser de diodo de 970 m sobre la morfología, la proliferación y la diferenciación de las células madre mesenquimales gingivalesspa
dc.titleEffects of 970 nm diode laser irradiation on morphology, proliferation, and dirrerentiation of gingival mesenchymal stem cellseng
dc.typeArticleeng
dc.typeArtículospa
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