Early evidence of the artificial transfer/transplant of mitochondria to oocytes and zygotes by MitoCeption

Simple item page
dc.coverageDOI: 10.1016/j.mito.2022.05.006
dc.creatorCabrera, Francisco
dc.creatorCastañeda, Verónica
dc.creatorMorales, Emilia
dc.creatorVelarde, Francesca
dc.creatorOrtega, Mayra
dc.creatorLeon-Sosa, Ariana
dc.creatorJorgensen, Christian
dc.creatorCaicedo, Andrés
dc.date2022
dc.date.accessioned2025-11-18T19:42:01Z
dc.date.available2025-11-18T19:42:01Z
dc.description<p>Oocytes may carry mutations in their mitochondrial DNA (mtDNA) which affect fertility and embryo development leading to hereditary diseases or rejection. Mitochondrial replacement therapies (MRTs) such as polar body transfer, spindle transfer and pronuclear transfer, aim to change dysfunctional to normal mitochondria inside oocytes and zygotes resulting in healthier offspring. Even with promising results, MRTs techniques are invasive to oocytes and may negatively affect their viability and the success of the procedure. This article shows early evidence of the use of MitoCeption, a mitochondria transfer/transplant (AMT/T) technique to possibly induce the internalization of exogenous mitochondria in a dose-dependent manner to recipient oocytes in comparison to coincubation. By using human isolated mitochondria in a mix obtained from different donors we were able to identify their mtDNA in murine oocytes by qPCR. Fluorescence microscopy showed that exogenous and transferred mitochondria (MitoTracker ® Red) by MitoCeption were internalized in oocytes and zygotes (CellTracker® Green). After maintaining mitocepted zygotes to two-cell embryos, we transferred them to subrogate female mice and obtained healthy mice pups; however, without clear evidence of the maintenance of human mtDNA in the tissues of mice pups. These early results are puzzling, and they open the path to generate more research regarding the use of MitoCeption in comparison to coincubation in order to transfer mitochondria to oocytes using less invasive procedures.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/d7b77efa-99ed-4c8c-b2ed-d8bee95e7a7b
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/52113
dc.languageeng
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourcevol.65 (2022) p.102-112
dc.subjectCoincubation
dc.subjectHeteroplasmy
dc.subjectMitoCeption
dc.subjectMitochondria replacement therapy (MRT)
dc.subjectMitochondrial disease
dc.subjectOocytes
dc.subjectXenogeneic transfer/transplant
dc.subjectZygotes
dc.subjectSDG 3 - Good Health and Well-being
dc.titleEarly evidence of the artificial transfer/transplant of mitochondria to oocytes and zygotes by MitoCeptioneng
dc.typeArticleeng
dc.typeArtículospa
Files
Collections
PURE