Proteomic Analysis of Salivary Extracellular Vesicles from COVID-19 Patients Reveals a Specific Anti-COVID-19 Response Protein Signature

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dc.coverageDOI: 10.3390/ijms25073704
dc.creatorWeber, Laura
dc.creatorTorres, Alfredo
dc.creatorRealini, Ornella
dc.creatorBendek, María José
dc.creatorMizgier, María Luisa
dc.creatorBrizuela, Claudia
dc.creatorHerrera, David
dc.creatorGonzález, Fermín E.
dc.creatorChaparro, Alejandra
dc.date2024
dc.date.accessioned2025-11-18T19:49:50Z
dc.date.available2025-11-18T19:49:50Z
dc.description<p>Despite the understanding of the coronavirus disease-19 (COVID-19), the role of salivary extracellular vesicles (sEVs) in COVID-19 remains unclear. Exploring the proteomic cargo of sEVs could prove valuable for diagnostic and prognostic purposes in assessing COVID-19. The proteomic cargo of sEVs from COVID-19(+) subjects and their healthy close contacts (HCC) was explored. sEVs were isolated by ultracentrifugation from unstimulated saliva samples, and subsequently characterized through nanoparticle tracking, transmission electron microscopy, and Western blot analyses. The proteomic cargo of sEVs was processed by LC-MS/MS. sEVs were morphologically compatible with EVs, with the presence of Syntenin-1 and CD81 EV markers. The sEV pellet showed 1417 proteins: 1288 in COVID-19(+) cases and 1382 in HCC. In total, 124 proteins were differentially expressed in sEVs from COVID-19(+) subjects. “Coronavirus-disease response”, “complement and coagulation cascades”, and “PMN extracellular trap formation” were the most enriched KEGG pathways in COVID-19(+) cases. The most represented biological processes were “Hemoglobin and haptoglobin binding” and “oxygen carrier activity”, and the best-denoted molecular functions were “regulated exocytosis and secretion” and “leucocyte and PMN mediated immunity”. sEV proteomic cargo in COVID-19(+) suggests activity related to immune response processes, oxygen transport, and antioxidant mechanisms. In contrast, in HCC, sEV signature profiles are mainly associated with epithelial homeostasis.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/c6295ac2-49ef-4c80-91a7-ffa17118fba9
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/56305
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.25 (2024) nr.7 p.1-20
dc.subjectCOVID-19
dc.subjectextracellular vesicles
dc.subjectproteomics
dc.subjectsaliva
dc.titleProteomic Analysis of Salivary Extracellular Vesicles from COVID-19 Patients Reveals a Specific Anti-COVID-19 Response Protein Signatureeng
dc.typeArticleeng
dc.typeArtículospa
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