Host-microbe computational proteomic landscape in oral cancer revealed key functional and metabolic pathways between Fusobacterium nucleatum and cancer progression

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dc.coverageDOI: 10.1038/s41368-024-00326-8
dc.creatorMuñoz-Grez, Camila Paz
dc.creatorVidal, Mabel Angélica
dc.creatorRojas, Tamara Beatriz
dc.creatorFerrada, Luciano Esteban
dc.creatorZuñiga, Felipe Andrés
dc.creatorVera, Agustin Andrés
dc.creatorSanhueza, Sergio Andrés
dc.creatorQuiroga, Romina Andrea
dc.creatorCabrera, Camilo Daniel
dc.creatorAntilef, Barbara Evelyn
dc.creatorCartes, Ricardo Andrés
dc.creatorAcevedo, Milovan Paolo
dc.creatorFraga, Marco Andrés
dc.creatorAlarcón-Zapata, Pedro Felipe
dc.creatorHernández, Mauricio Alejandro
dc.creatorSalas-Burgos, Alexis Marcelo
dc.creatorTapia-Belmonte, Francisco
dc.creatorYáñez, Milly Loreto
dc.creatorRiquelme, Erick Marcelo
dc.creatorGonzález, Wilfredo Alejandro
dc.creatorRivera, Cesar Andrés
dc.creatorOñate, Angel Alejandro
dc.creatorLamperti, Liliana Ivonne
dc.creatorNova-Lamperti, Estefanía
dc.date2025
dc.date.accessioned2025-11-18T19:44:30Z
dc.date.available2025-11-18T19:44:30Z
dc.description<p>Oral squamous cell carcinoma (OSCC) is the most common manifestation of oral cancer. It has been proposed that periodontal pathogens contribute to OSCC progression, mainly by their virulence factors. However, the main periodontal pathogen and its mechanism to modulate OSCC cells remains not fully understood. In this study we investigate the main host-pathogen pathways in OSCC by computational proteomics and the mechanism behind cancer progression by the oral microbiome. The main host-pathogen pathways were analyzed in the secretome of biopsies from patients with OSCC and healthy controls by mass spectrometry. Then, functional assays were performed to evaluate the host-pathogen pathways highlighted in oral cancer. Host proteins associated with LPS response, cell migration/adhesion, and metabolism of amino acids were significantly upregulated in the human cancer proteome, whereas the complement cascade was downregulated in malignant samples. Then, the microbiome analysis revealed large number and variety of peptides from Fusobacterium nucleatum (F. nucleatum) in OSCC samples, from which several enzymes from the L-glutamate degradation pathway were found, indicating that L-glutamate from cancer cells is used as an energy source, and catabolized into butyrate by the bacteria. In fact, we observed that F. nucleatum modulates the cystine/glutamate antiporter in an OSCC cell line by increasing SLC7A11 expression, promoting L-glutamate efflux and favoring bacterial infection. Finally, our results showed that F. nucleatum and its metabolic derivates promote tumor spheroids growth, spheroids-derived cell detachment, epithelial-mesenchymal transition and Galectin-9 upregulation. Altogether, F. nucleatum promotes pro-tumoral mechanism in oral cancer.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/465cec11-d811-4c32-abef-c448d5667c0f
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/53430
dc.languageeng
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourcevol.17 (2025) date: 2025-01-02 nr.1 p.1
dc.subjectHumans
dc.subjectFusobacterium nucleatum/metabolism
dc.subjectMouth Neoplasms/microbiology
dc.subjectDisease Progression
dc.subjectProteomics
dc.subjectCarcinoma, Squamous Cell/microbiology
dc.subjectHost-Pathogen Interactions
dc.subjectMetabolic Networks and Pathways
dc.subjectCase-Control Studies
dc.subjectMass Spectrometry
dc.subjectSDG 3 - Good Health and Well-being
dc.titleHost-microbe computational proteomic landscape in oral cancer revealed key functional and metabolic pathways between Fusobacterium nucleatum and cancer progressioneng
dc.typeArticleeng
dc.typeArtículospa
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