Mesenchymal Stem Cells Derived from Human Inflamed Dental Pulp Exhibit Impaired Immunomodulatory Capacity In Vitro

Simple item page
dc.coverageDOI: 10.1016/j.joen.2020.05.003
dc.creatorInostroza, Carolina
dc.creatorVega-Letter, Ana María
dc.creatorBrizuela, Claudia
dc.creatorCastrillón, Luis
dc.creatorSaint Jean, Nicole
dc.creatorDuran, Carol Mira
dc.creatorCarrión, Flavio
dc.date2020
dc.date.accessioned2025-11-18T19:47:32Z
dc.date.available2025-11-18T19:47:32Z
dc.description<p>Introduction: Dental pulp stem cells (DPSC) are very attractive in regenerative medicine. In this study, we focused on the characterization of the functional properties of mesenchymal stem cells derived from DPSCs. Currently, it is unknown whether inflammatory conditions present in an inflamed dental pulp tissue could alter the immunomodulatory properties of DPSCs. This study aimed to evaluate the immunomodulatory capacity in vitro of DPSCs derived from healthy and inflamed dental pulp. Methods: DPSCs from 10 healthy and inflamed dental pulps (irreversible pulpitis) were characterized according to the minimal criteria of the International Society for Cell Therapy, proliferation, differential potential, and colony-forming units. Furthermore, the immunomodulatory capacity of DPSCs was tested on the proliferation of T lymphocytes by flow cytometry and the in vitro enzyme activity of indoleamine 2, 3-dioxygenase. Results: There were no significant differences in the DPSC characteristics and properties such as immunophenotype, tridifferentiation, colony-forming units, and proliferation of the DPSCs derived from normal and inflamed pulp tissue. Furthermore, there were significant differences in the immunomodulatory capacity of DPSCs obtained from human healthy dental pulp and with the diagnosis of irreversible pulpitis. Conclusions: Our results showed that DPSCs isolated from inflamed dental pulp showed typical characteristics of MSCs and diminished immunosuppressive capacity in vitro in comparison with MSCs derived from healthy dental pulp. Further investigation in vivo is needed to clarify the mechanism of this diminished immunosuppressive capacity.</p>eng
dc.descriptionIntroduction: Dental pulp stem cells (DPSC) are very attractive in regenerative medicine. In this study, we focused on the characterization of the functional properties of mesenchymal stem cells derived from DPSCs. Currently, it is unknown whether inflammatory conditions present in an inflamed dental pulp tissue could alter the immunomodulatory properties of DPSCs. This study aimed to evaluate the immunomodulatory capacity in vitro of DPSCs derived from healthy and inflamed dental pulp. Methods: DPSCs from 10 healthy and inflamed dental pulps (irreversible pulpitis) were characterized according to the minimal criteria of the International Society for Cell Therapy, proliferation, differential potential, and colony-forming units. Furthermore, the immunomodulatory capacity of DPSCs was tested on the proliferation of T lymphocytes by flow cytometry and the in vitro enzyme activity of indoleamine 2, 3-dioxygenase. Results: There were no significant differences in the DPSC characteristics and properties such as immunophenotype, tridifferentiation, colony-forming units, and proliferation of the DPSCs derived from normal and inflamed pulp tissue. Furthermore, there were significant differences in the immunomodulatory capacity of DPSCs obtained from human healthy dental pulp and with the diagnosis of irreversible pulpitis. Conclusions: Our results showed that DPSCs isolated from inflamed dental pulp showed typical characteristics of MSCs and diminished immunosuppressive capacity in vitro in comparison with MSCs derived from healthy dental pulp. Further investigation in vivo is needed to clarify the mechanism of this diminished immunosuppressive capacity. © 2020 American Association of Endodontistsspa
dc.identifierhttps://investigadores.uandes.cl/en/publications/22662980-ecbf-407c-91dd-9bba91e545a7
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/55096
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.46 (2020) nr.8 p.1091-1098.e2
dc.subjectHealthy dental pulp
dc.subjectinflamed dental pulp
dc.subjectmesenchymal stem cells
dc.subjectpulpitis
dc.subjectSDG 3 - Good Health and Well-being
dc.titleMesenchymal Stem Cells Derived from Human Inflamed Dental Pulp Exhibit Impaired Immunomodulatory Capacity In Vitroeng
dc.typeArticleeng
dc.typeArtículospa
Files
Collections
PURE