Method for measurement of collagen monomer orientation in fluorescence microscopy

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dc.coverageDOI: 10.1117/12.2582586
dc.creatorAlzola, Rodrigo P.
dc.creatorDelpiano, Jose
dc.creatorGajjar, Anuj
dc.creatorStureborg, Rickard
dc.creatorSiadat, Seyed Mohammad
dc.creatorRuberti, Jeffrey W.
dc.creatorDimarzio, Charles A.
dc.date2021
dc.date.accessioned2025-11-18T19:41:09Z
dc.date.available2025-11-18T19:41:09Z
dc.description<p>Collagen is one of the most important proteins in mammals, conforming most animal tissues. This work explores how a basic collagen monomer unit is visualized using fluorescence microscopy and how its spatial orientation is determined. Defining the orientation of collagen monomers is not a trivial problem, as the particle has a weak contrast and is relatively small. Possible attach fluorescence tags for contrast, but the size is still a problem for detecting orientation using fluorescence microscopy. This document presents a simulation of the visualization of collagen monomers and two methods for detecting monomer and classifying its orientation. A modify Gabor filter set, and an automatic classifier, trained by convolutional neuronal network (CNN), were used. By evaluating the performance of these two approaches compare to human observation, our results show that it is possible to determine the location and orientation of a single monomer with fluorescence microscopy. These findings can contribute to understanding collagen elements as collagen fibril.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/25ac5102-b51d-4845-94a3-64fc5af7e2b0
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/51669
dc.languageeng
dc.publisherSPIE
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourceBrown, Thomas G., Wilson, Tony, Waller, Laura (Ed.), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, p.19. SPIE. [ISBN 9781510641334]
dc.subjectCollagen
dc.subjectcomputer vision
dc.subjectfluorescence microscopy
dc.subjectmachine learning
dc.titleMethod for measurement of collagen monomer orientation in fluorescence microscopyeng
dc.typeChaptereng
dc.typeCapítulospa
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