Molecular signatures of extracellular vesicles in oral fluids of periodontitis patients

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dc.coverageDOI: 10.1111/odi.13338
dc.creatorChaparro Padilla, Alejandra
dc.creatorWeber Aracena, Laura
dc.creatorRealini Fuentes, Ornella
dc.creatorAlbers Busquetts, Daniela
dc.creatorHernández Ríos, Marcela
dc.creatorRamírez Lobos, Valeria
dc.creatorPascual La Rocca, Andrés
dc.creatorNart Molina, José
dc.creatorBeltrán Varas, Victor
dc.creatorAcuña-Gallardo, Stephanie
dc.creatorSanz Ruiz, Antonio
dc.date2020
dc.date.accessioned2025-11-18T19:47:08Z
dc.date.available2025-11-18T19:47:08Z
dc.description<p>Objective: To characterize extracellular vesicles (EVs) in gingival crevicular fluid (GCF) and saliva samples from healthy/gingivitis and periodontitis patients and correlate them with clinical inflammatory periodontal parameters. Material and Method: An exploratory study, including 86 subjects, was conducted. Clinical and periodontal data were recorded, and oral fluid samples were obtained. EVs were precipitated by ExoQuick-TC™ and characterized by nanoparticle tracking (NanoSight™), Western blot (WB), transmission electron microscopy (TEM), and ELISA analysis. Results: TEM showed nanoparticles morphologically compatible with EVs, and WB analysis revealed bands of specific EV markers (CD9, TSG101, and Alix) in both oral fluids of periodontitis and healthy/gingivitis subjects. The total concentration of EVs in GCF was increased in periodontitis patients compared to healthy/gingivitis subjects (p =.017). However, we did not observe differences in the EV concentration of saliva samples (p =.190). The size of GCF-EVs was 144.2 nm in periodontitis and 160.35 nm in healthy/gingivitis patients (p =.038). The CD63 exosome marker was increased in GCF of periodontitis patients (p =.00001). The total concentration of EVs in GCF was correlated with bleeding on probing (rho = 0.63, p =.002), periodontal probing depth (rho = 0.56, p =.009), and clinical attachment level (rho = 0.48, p =.030). Conclusion: Periodontitis patients have an increased concentration of EVs in GCF, and their role in periodontitis should be clarified.</p>eng
dc.descriptionObjective<br/>To characterize extracellular vesicles (EVs) in gingival crevicular fluid (GCF) and saliva samples from healthy/gingivitis and periodontitis patients and correlate them with clinical inflammatory periodontal parameters.<br/><br/>Material and Method<br/>An exploratory study, including 86 subjects, was conducted. Clinical and periodontal data were recorded, and oral fluid samples were obtained. EVs were precipitated by ExoQuick-TC™ and characterized by nanoparticle tracking (NanoSight™), Western blot (WB), transmission electron microscopy (TEM), and ELISA analysis.<br/><br/>Results<br/>TEM showed nanoparticles morphologically compatible with EVs, and WB analysis revealed bands of specific EV markers (CD9, TSG101, and Alix) in both oral fluids of periodontitis and healthy/gingivitis subjects. The total concentration of EVs in GCF was increased in periodontitis patients compared to healthy/gingivitis subjects (p = .017). However, we did not observe differences in the EV concentration of saliva samples (p = .190). The size of GCF-EVs was 144.2 nm in periodontitis and 160.35 nm in healthy/gingivitis patients (p = .038). The CD63 exosome marker was increased in GCF of periodontitis patients (p = .00001). The total concentration of EVs in GCF was correlated with bleeding on probing (rho = 0.63, p = .002), periodontal probing depth (rho = 0.56, p = .009), and clinical attachment level (rho = 0.48, p = .030).<br/><br/>Conclusion<br/>Periodontitis patients have an increased concentration of EVs in GCF, and their role in periodontitis should be clarified.spa
dc.identifierhttps://investigadores.uandes.cl/en/publications/606ee136-bc89-4002-a392-79d9e3cb47d0
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/54885
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.26 (2020) date: 2020-09-01 nr.6 p.1318-1325
dc.subjectexosomes
dc.subjectextracellular vesicles
dc.subjectmicro-vesicles
dc.subjectperiodontitis
dc.titleMolecular signatures of extracellular vesicles in oral fluids of periodontitis patientseng
dc.typeArticleeng
dc.typeArtículospa
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