Benzimidazole-Derived B2 as a Fluorescent Probe for Bacterial Outer Membrane Vesicle (OMV) Labeling: Integrating DFT, Molecular Dynamics, Flow Cytometry, and Confocal Microscopy

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dc.coverageDOI: 10.3390/ijms26104682
dc.creatorParra, Francisco
dc.creatorCarreño, Alexander
dc.creatorAncede-Gallardo, Evys
dc.creatorMajluf, Diana
dc.creatorSoto, Jorge A.
dc.creatorSepúlveda, Romina V.
dc.creatorAguayo, Daniel
dc.creatorOtero, María Carolina
dc.creatorCalderón, Iván L.
dc.creatorGil, Fernando
dc.creatorFuentes, Juan A.
dc.date2025
dc.date.accessioned2026-01-05T21:22:11Z
dc.date.available2026-01-05T21:22:11Z
dc.description<p>Bacterial outer membrane vesicles (OMVs) are nanoscale extracellular structures produced by Gram-negative bacteria that are critical for microbial biology and host-pathogen interactions and have great potential in biotechnological applications. Despite the availability of fluorescent dyes for OMV studies, many are repurposed from eukaryotic extracellular vesicle research and are not explicitly optimized for OMVs, leading to challenges in achieving consistent labeling, minimizing background noise, and preserving vesicle integrity during analyses. This study evaluates B2, a benzimidazole-derived fluorophore, for OMV labeling in advanced techniques like flow cytometry and confocal microscopy. OMVs were isolated from Escherichia coli strains BL21 and O157, and their integrity was confirmed using transmission electron microscopy (TEM). B2 staining protocols were optimized for OMVs, and fluorescence analyses revealed specific interactions with the vesicle membranes, reducing aggregation and enhancing signal uniformity. Flow cytometry indicated near-complete labeling efficiency (98–100%) with minimal background interference. Confocal microscopy further validated B2’s effectiveness, showing evident OMV internalization into epithelial HT-29 cells and compatibility with other fluorophores. Density functional theory (DFT) calculations, including Fukui function analysis, identified key electrophilic and nucleophilic regions in B2 that facilitate specific hydrogen bonding and polar interactions with membrane components. Non-covalent interaction (NCI) analysis revealed pronounced intramolecular hydrogen bonding along with discrete regions of weak van der Waals interactions. Molecular dynamics simulations suggest that B2 exhibits an affinity for both the hydrophobic core of the lipid bilayer and the core oligosaccharide region of the LPS layer, which collectively ensures sustained retention of the dye. The findings presented in this study position B2 as a valuable fluorophore for OMV research.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/b72a9d68-90ec-4d84-954d-c327969ecca8
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/69765
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.26 (2025) nr.10
dc.subjectOMVs
dc.subjectbenzimidazole compound B2
dc.subjectconfocal microscopy
dc.subjectflow cytometry
dc.subjectfluorescent dyes
dc.subjectfluorescent labeling of OMVs
dc.subjectintramolecular hydrogen bond
dc.subjectouter membrane vesicles
dc.titleBenzimidazole-Derived B2 as a Fluorescent Probe for Bacterial Outer Membrane Vesicle (OMV) Labeling: Integrating DFT, Molecular Dynamics, Flow Cytometry, and Confocal Microscopyeng
dc.typeArticleeng
dc.typeArtículospa
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