A Quinoa Protein Hydrolysate Fractionated by Electrodialysis with Ultrafiltration Membranes Improves Maternal and Fetal Outcomes in a Mouse Model of Gestational Diabetes Mellitus

Simple item page
dc.coverageDOI: 10.1002/mnfr.202300047
dc.creatorBusso, Dolores
dc.creatorGonzález, Adrián
dc.creatorSantander, Nicolás
dc.creatorSaavedra, Fujiko
dc.creatorQuiroz, Alonso
dc.creatorRivera, Katherine
dc.creatorGonzález, Javier
dc.creatorOlmos, Pablo
dc.creatorMarette, André
dc.creatorBazinet, Laurent
dc.creatorIllanes, Sebastián
dc.creatorEnrione, Javier
dc.date2023
dc.date.accessioned2025-11-18T19:49:24Z
dc.date.available2025-11-18T19:49:24Z
dc.description<p>Scope: Quinoa intake exerts hypoglycemic and hypolipidemic effects in animals and humans. Although peptides from quinoa inhibit key enzymes involved in glucose homeostasis in vitro, their in vivo antidiabetic properties have not been investigated. Methods and results: This study evaluated the effect of oral administration of a quinoa protein hydrolysate (QH) produced through enzymatic hydrolysis and fractionation by electrodialysis with ultrafiltration membrane (EDUF) (FQH) on the metabolic and pregnancy outcomes of Lepdb/+ pregnant mice, a preclinical model of gestational diabetes mellitus. The 4-week pregestational consumption of 2.5 mg mL<sup>−1</sup> of QH in water prevented glucose intolerance and improves hepatic insulin signaling in dams, also reducing fetal weights. Sequencing and bioinformatic analyses of the defatted FQH (FQHD) identified 11 peptides 6–10 amino acids long that aligned with the quinoa proteome and exhibited putative anti-dipeptidyl peptidase-4 (DPP-IV) activity, confirmed in vitro in QH, FQH, and FDQH fractions. Peptides homologous to mouse and human proteins enriched for biological processes related to glucose metabolism are also identified. Conclusion: Processing of quinoa protein may be used to develop a safe and effective nutritional intervention to control glucose intolerance during pregnancy. Further studies are required to confirm if this nutritional intervention is applicable to pregnant women.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/a362c062-ad9f-4ec8-8ff7-040bbb752cff
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/56071
dc.languageeng
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourcevol.67 (2023) nr.21
dc.subjectelectrodialysis with filtration membrane
dc.subjectgestational diabetes mellitus
dc.subjectmice
dc.subjectpeptides
dc.subjectprotein hydrolysate
dc.subjectquinoa
dc.subjectSDG 3 - Good Health and Well-being
dc.titleA Quinoa Protein Hydrolysate Fractionated by Electrodialysis with Ultrafiltration Membranes Improves Maternal and Fetal Outcomes in a Mouse Model of Gestational Diabetes Mellituseng
dc.typeArticleeng
dc.typeArtículospa
Files
Collections
PURE