Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP

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dc.coverageDOI: 10.1016/j.biortech.2022.127141
dc.creatorYáñez, Luz
dc.creatorRodríguez, Yadira
dc.creatorScott, Felipe
dc.creatorVergara-Fernández, Alberto
dc.creatorMuñoz, Raúl
dc.date2022
dc.date.accessioned2025-11-18T19:54:29Z
dc.date.available2025-11-18T19:54:29Z
dc.description<p>Methylocystis parvus OBBP accumulates polyhydroxybutyrate (PHB) using methane as the sole carbon and energy source. In this work, the feasibility of producing (R)-3-hydroxybutyric acid (R3HBA) via intracellularly accumulated PHB through depolymerization (in-vivo) was investigated. Results showed that a PHB to R3HBA conversion of 77.2 ± 0.9% (R3HBA titer of 0.153 ± 0.002 g L<sup>-1</sup>) can be attained in a mineral medium containing 1 g L<sup>-1</sup> KNO<sub>3</sub> at 30 °C with shaking at 200 rpm and a constant pH of 11 for 72 h. Nitrogen deprivation and neutral or acidic pHs strongly reduced the excreted R3HBA concentration. Reduced oxygen availability negatively affected the R3HBA yield, which decreased to 73.6 ± 4.9% (titer of 0.139 ± 0.01 g L<sup>-1</sup>) under microaerobic conditions. Likewise, the presence of increasing concentrations of R3HBA in the medium before the onset of PHB depolymerization reduced the initial R3HBA release rate and R3HBA yield.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/9ab24c72-fb33-42f8-8aab-db2beef65dca
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/58788
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.353 (2022) p.127141
dc.subject(R)-3-hydroxybutyrate
dc.subjectChiral compounds
dc.subjectGreenhouse gas valorization
dc.subjectMethanotrophic bacteria
dc.subjectPolyhydroxybutyrate
dc.titleProduction of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBPeng
dc.typeArticleeng
dc.typeArtículospa
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