Oral extracellular vesicles in early pregnancy can identify patients at risk of developing gestational diabetes mellitus

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dc.coverageDOI: 10.1371/journal.pone.0218616
dc.creatorMonteiro, Lara J.
dc.creatorVaras-Godoy, Manuel
dc.creatorMonckeberg, Max
dc.creatorRealini, Ornella
dc.creatorHernández, Marcela
dc.creatorRice, Gregory
dc.creatorRomero, Roberto
dc.creatorSaavedra, José F.
dc.creatorIllanes, Sebastián E.
dc.creatorChaparro, Alejandra
dc.date2018
dc.date.accessioned2025-11-18T19:40:32Z
dc.date.available2025-11-18T19:40:32Z
dc.description<p>Aim To isolate and characterize oral extracellular vesicles from gingival crevicular fluid at 11–14 weeks and evaluate their capacity to identify patients at risk of developing gestational diabetes mellitus. Methods A case-control study was conducted, including patients who developed gestational diabetes mellitus (n = 11) and healthy pregnant controls (n = 23). Obstetric and periodontal histories were recorded at 11–14 weeks of gestation, and samples of gingival crevicular fluid obtained. Extracellular vesicles were isolated from gingival crevicular fluid by ExoQuick. Nanoparticle tracking analysis, ELISA and transmission electron microscopy were used to characterize extracellular vesicles. Results Total extracellular vesicles isolated from gingival crevicular fluid were significantly higher in patients who developed gestational diabetes mellitus later in pregnancy compared to normoglycemic pregnant women (6.3x10<sup>9</sup> vs 1.7 x10<sup>10</sup>, p value = 0.0026), and the concentration of the extracellular vesicles delivered an area under the ROC curve of 0.81. The distribution size of extracellular vesicles obtained using ExoQuick was around 148 ± 57 nm. There were no significant differences in the periodontal status between cases and controls. The exosome transmembrane protein CD63 was also detected in the extracellular vesicles of gingival crevicular fluid. Conclusion We were able to isolate extracellular vesicles from gingival crevicular fluid using a method that is suitable to be applied in a clinical setting. Our results provide an insight into the potential capacity of first trimester oral extracellular vesicles as early biomarkers for the prediction of gestational diabetes mellitus in pre-symptomatic women.</p>eng
dc.descriptionAim To isolate and characterize oral extracellular vesicles from gingival crevicular fluid at 11–14 weeks and evaluate their capacity to identify patients at risk of developing gestational diabetes mellitus. Methods A case-control study was conducted, including patients who developed gestational diabetes mellitus (n = 11) and healthy pregnant controls (n = 23). Obstetric and periodontal histories were recorded at 11–14 weeks of gestation, and samples of gingival crevicular fluid obtained. Extracellular vesicles were isolated from gingival crevicular fluid by ExoQuick. Nanoparticle tracking analysis, ELISA and transmission electron microscopy were used to characterize extracellular vesicles. Results Total extracellular vesicles isolated from gingival crevicular fluid were significantly higher in patients who developed gestational diabetes mellitus later in pregnancy compared to normoglycemic pregnant women (6.3x109 vs 1.7 x1010, p value = 0.0026), and the concentration of the extracellular vesicles delivered an area under the ROC curve of 0.81. The distribution size of extracellular vesicles obtained using ExoQuick was around 148 ± 57 nm. There were no significant differences in the periodontal status between cases and controls. The exosome transmembrane protein CD63 was also detected in the extracellular vesicles of gingival crevicular fluid. Conclusion We were able to isolate extracellular vesicles from gingival crevicular fluid using a method that is suitable to be applied in a clinical setting. Our results provide an insight into the potential capacity of first trimester oral extracellular vesicles as early biomarkers for the prediction of gestational diabetes mellitus in pre-symptomatic women. Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.spa
dc.identifierhttps://investigadores.uandes.cl/en/publications/ce5dc9aa-dbf3-4968-bd3d-3ad00d02c532
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/51333
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.14 (2018) date: 2018-06-01 nr.6
dc.subjectAdult
dc.subjectCase-Control Studies
dc.subjectDiabetes
dc.subjectGestational
dc.subjectxtracellular Vesicles
dc.subjectFemale
dc.subjectGingival Crevicular Fluid
dc.subjectHumans
dc.subjectLiquid Biopsy
dc.subjectParticle Size
dc.subjectPeriodontitis
dc.subjectPregnancy
dc.subjectPregnancy Trimester
dc.subjectFirst
dc.subjectRetrospective Studies
dc.subjectRisk Factors
dc.subjectTetraspanin 30
dc.subjectSDG 3 - Good Health and Well-being
dc.titleOral extracellular vesicles in early pregnancy can identify patients at risk of developing gestational diabetes mellituseng
dc.typeArticleeng
dc.typeArtículospa
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