A Psychrophilic GelMA: Breaking Technical and Immunological Barriers for Multimaterial High-Resolution 3D Bioprinting

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dc.coverageDOI: 10.1021/acs.biomac.2c01019
dc.creatorZaupa, Alessandro
dc.creatorTerraza, Claudia
dc.creatorAbarzúa-Illanes, Phammela N.
dc.creatorByres, Nicholas
dc.creatorZavala, Gabriela
dc.creatorCuenca, Jimena
dc.creatorHidalgo, Carmen
dc.creatorViafara-Garcia, Sergio M.
dc.creatorWolf, Bettina
dc.creatorPino-Lagos, Karina
dc.creatorBlaker, Jonny J.
dc.creatorRumbak, Mayan
dc.creatorKhoury, Maroun
dc.creatorEnrione, Javier
dc.creatorAcevedo, Juan Pablo
dc.date2022
dc.date.accessioned2025-11-18T19:42:18Z
dc.date.available2025-11-18T19:42:18Z
dc.description<p>The increasing demand for tissue replacement has encouraged scientists worldwide to focus on developing new biofabrication technologies. Multimaterials/cells printed with stringent resolutions are necessary to address the high complexity of tissues. Advanced inkjet 3D printing can use multimaterials and attain high resolution and complexity of printed structures. However, a decisive yet limiting aspect of translational 3D bioprinting is selecting the befitting material to be used as bioink; there is a complete lack of cytoactive bioinks with adequate rheological, mechanical, and reactive properties. This work strives to achieve the right balance between resolution and cell support through methacrylamide functionalization of a psychrophilic gelatin and new fluorosurfactants used to engineer a photo-cross-linkable and immunoevasive bioink. The syntonized parameters following optimal formulation conditions allow proficient printability in a PolyJet 3D printer comparable in resolution to a commercial synthetic ink (∼150 μm). The bioink formulation achieved the desired viability (∼80%) and proliferation of co-printed cells while demonstrating in vivo immune tolerance of printed structures. The practical usage of existing high-resolution 3D printing systems using a novel bioink is shown here, allowing 3D bioprinted structures with potentially unprecedented complexity.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/7a5a257a-1b0c-4937-9da6-cc8801fc7d16
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/52277
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.24 (2022) nr.1 p.150-165
dc.titleA Psychrophilic GelMA: Breaking Technical and Immunological Barriers for Multimaterial High-Resolution 3D Bioprintingeng
dc.typeArticleeng
dc.typeArtículospa
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