Two internal bottlenecks cause the overflow metabolism leading to poly(3-hydroxybutyrate) production in Azohydromonas lata DSM1123

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dc.coverageDOI: 10.1016/j.jece.2021.105665
dc.creatorScott, Felipe Ignacio
dc.creatorYañez, Luz
dc.creatorConejeros, Raúl
dc.creatorAraya, Blanca
dc.creatorVergara, Alberto
dc.date2021
dc.date.accessioned2025-11-18T19:41:06Z
dc.date.available2025-11-18T19:41:06Z
dc.description<p>Polyhydroxybutyrate production in the bacteria Azohydromonas lata DSM1123 has been regarded as growth-associated based on batch experiments. However, growth association can only be confirmed under chemostat culture. In this work, an experimental approach followed by Flux Balance Analysis (FBA) modeling was used to analyze the behavior of this strain under glucose, oxygen, and nitrogen-limited conditions. The model allowed the identification of candidate enzymes constraining the flux through the tricarboxylic acid cycle, and the role of ATP synthase as the key flux controlling enzyme for the respiratory metabolism, in terms of its share of the total metabolically active protein. The results presented in this work and the model developed could assist in the economic and environmental assessment of new continuous processes to produce PHB and other metabolites in A. lata DSM1123, a strain capable of hydrogen use and CO2 fixation.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/968b9899-0214-4f67-8eb7-a125c8015dd7
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/51639
dc.languageeng
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourcevol.9 (2021) date: 2021-08-01 nr.4
dc.subjectAzohydromonas lata
dc.subjectBiopolymers
dc.subjectFlux balance analysis
dc.subjectOverflow metabolism
dc.subjectPolyhydroxybutyrate
dc.titleTwo internal bottlenecks cause the overflow metabolism leading to poly(3-hydroxybutyrate) production in Azohydromonas lata DSM1123eng
dc.typeArticleeng
dc.typeArtículospa
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