Gut Microbiota-Derived Extracellular Vesicles Influence Alcohol Intake Preferences in Rats

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dc.coverageDOI: 10.1002/jev2.70059
dc.creatorDíaz-Ubilla, Macarena
dc.creatorFigueroa-Valdés, Aliosha I.
dc.creatorTobar, Hugo E.
dc.creatorQuintanilla, María Elena
dc.creatorDíaz, Eugenio
dc.creatorMorales, Paola
dc.creatorBerríos-Cárcamo, Pablo
dc.creatorSantapau, Daniela
dc.creatorGallardo, Javiera
dc.creatorde Gregorio, Cristian
dc.creatorUgalde, Juan
dc.creatorRojas, Carolina
dc.creatorGonzalez-Madrid, Antonia
dc.creatorEzquer, Marcelo
dc.creatorIsrael, Yedy
dc.creatorAlcayaga-Miranda, Francisca
dc.creatorEzquer, Fernando
dc.date2025
dc.date.accessioned2025-11-18T19:44:13Z
dc.date.available2025-11-18T19:44:13Z
dc.description<p>Growing preclinical and clinical evidence suggests a link between gut microbiota dysbiosis and problematic alcohol consumption. Extracellular vesicles (EVs) are key mediators involved in bacteria-to-host communication. However, their potential role in mediating addictive behaviour remains unexplored. This study investigates the role of gut microbiota-derived bacterial extracellular vesicles (bEVs) in driving high alcohol consumption. bEVs were isolated from the gut microbiota of a high alcohol-drinking rat strain (UChB rats), either ethanol-naïve or following chronic alcohol consumption and administered intraperitoneally or orally to alcohol-rejecting male and female Wistar rats. Both types of UChB-derived bEVs increased Wistar's voluntary alcohol consumption (three bottle choice test) up to 10-fold (p &lt; 0.0001), indicating that bEVs are able and sufficient to transmit drinking behaviour across different rat strains. Molecular analysis revealed that bEVs administration did not induce systemic or brain inflammation in the recipient animals, suggesting that the increased alcohol intake triggered by UChB-derived bEVs operates through an inflammation-independent mechanism. Furthermore, we demonstrate that the vagus nerve mediates the bEV-induced increase in alcohol consumption, as bilateral vagotomy completely abolished the high drinking behaviour induced by both intraperitoneally injected and orally administered bEVs. Thus, this study identifies bEVs as a novel mechanism underlying gut microbiota-induced high alcohol intake in a vagus nerve-dependent manner.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/7ce3468e-1cf2-4d97-8576-ebbd08c78ff6
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/53301
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.14 (2025) nr.3
dc.subjectaddictive behaviour
dc.subjectalcohol consumption
dc.subjectbEVs
dc.subjectbacterial vesicles
dc.subjectgut microbiota
dc.subjectinflammation
dc.subjectmicrobiota-derived EVs
dc.subjectvagus nerve
dc.titleGut Microbiota-Derived Extracellular Vesicles Influence Alcohol Intake Preferences in Ratseng
dc.typeArticleeng
dc.typeArtículospa
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