Aminoguanidine prevents the oxidative stress, inhibiting elements of inflammation, endothelial activation, mesenchymal markers, and confers a renoprotective effect in renal ischemia and reperfusion injury

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dc.coverageDOI: 10.3390/antiox10111724
dc.creatorPasten, Consuelo
dc.creatorLozano, Mauricio
dc.creatorRocco, Jocelyn
dc.creatorCarrión, Flavio
dc.creatorAlvarado, Cristobal
dc.creatorLiberona, Jéssica
dc.creatorMichea, Luis
dc.creatorIrarrázabal, Carlos E.
dc.date2021
dc.date.accessioned2025-11-18T19:48:15Z
dc.date.available2025-11-18T19:48:15Z
dc.description<p>Oxidative stress produces macromolecules dysfunction and cellular damage. Renal ische-mia‐reperfusion injury (IRI) induces oxidative stress, inflammation, epithelium and endothelium damage, and cessation of renal function. The IRI is an inevitable process during kidney transplan-tation. Preliminary studies suggest that aminoguanidine (AG) is an antioxidant compound. In this study, we investigated the antioxidant effects of AG (50 mg/kg, intraperitoneal) and its association with molecular pathways activated by IRI (30 min/48 h) in the kidney. The antioxidant effect of AG was studied measuring GSSH/GSSG ratio, GST activity, lipoperoxidation, iNOS, and Hsp27 levels. In addition, we examined the effect of AG on elements associated with cell survival, inflammation, endothelium, and mesenchymal transition during IRI. AG prevented lipid peroxidation, increased GSH levels, and recovered the GST activity impaired by IRI. AG was associated with inhibition of iNOS, Hsp27, endothelial activation (VE‐cadherin, PECAM), mesenchymal markers (vimentin, fas-cin, and HSP47), and inflammation (IL‐1β, IL‐6, Foxp3, and IL‐10) upregulation. In addition, AG reduced kidney injury (NGAL, clusterin, Arg‐2, and TFG‐β1) and improved kidney function (glo-merular filtration rate) during IRI. In conclusion, we found new evidence of the antioxidant properties of AG as a renoprotective compound during IRI. Therefore, AG is a promising compound to treat the deleterious effect of renal IRI.</p>eng
dc.identifierhttps://investigadores.uandes.cl/en/publications/af94b73a-a76d-4986-b876-abddb8867be0
dc.identifier.urihttps://repositorio.uandes.cl/handle/uandes/55463
dc.languageeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourcevol.10 (2021) nr.11
dc.subjectAminoguanidine
dc.subjectAntioxidants
dc.subjectIschemia‐reperfusion injury
dc.subjectOxidative stress
dc.subjectRenal protection
dc.titleAminoguanidine prevents the oxidative stress, inhibiting elements of inflammation, endothelial activation, mesenchymal markers, and confers a renoprotective effect in renal ischemia and reperfusion injuryeng
dc.typeArticleeng
dc.typeArtículospa
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